ArticleGenes & development2000
Yeast heterochromatin is a dynamic structure that requires silencers continuously.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 134 citations in OpenAlex.
- Twisting the End Game: How Telomere Chromatin Modifications Shape Telomere Maintenance.Aging cell · 2026Review
- Sir proteins impede, but do not prevent, access to silent chromatin in living Saccharomyces cerevisiae.Scientific reports · 2026Article
- Replication-coupled inheritance of chromatin states.Cell insight · 2024Review
- Transcriptional silencing in Saccharomyces cerevisiae: known unknowns.Epigenetics & chromatin · 2024Review
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Yeast heterochromatin stably silences only weak regulatory elements by altering burst duration.Cell reports · 2024Article
- Minimal requirements for the epigenetic inheritance of engineered silent chromatin domains.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Interplay between epigenome and 3D chromatin structure.BMB reports · 2023Review
- Yeast Heterochromatin Only Stably Silences Weak Regulatory Elements by Altering Burst Duration.bioRxiv : the preprint server for biology · 2023Article
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- Live analysis of position-effect variegation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Measuring the buffering capacity of gene silencing inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin.Molecular cell · 2021Article
- Nucleosome Positioning Regulates the Establishment, Stability, and Inheritance of Heterochromatin inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Sir2 mitigates an intrinsic imbalance in origin licensing efficiency between early- and late-replicating euchromatin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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- Monitoring of switches in heterochromatin-induced silencing shows incomplete establishment and developmental instabilities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Yeast epigenetics: the inheritance of histone modification states.Bioscience reports · 2019Review
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- Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptional silencing of the HM loci in yeast requires cis-acting elements, termed silencers, that function during S-phase passage to establish the silent state. To study the role of the regulatory elements in maintenance of repression, site-specific recombination was used to uncouple preassembled silent chromatin fragments from silencers. DNA rings excised from HMR were initially silent but ultimately reactivated, even in G(1)- or G(2)/M-arrested cells. In contrast, DNA rings bearing HML-derived sequence were stably repressed due to the presence of a protosilencing element. These data show that silencers (or protosilencers) are required continuously for maintenance of silent chromatin. Reactivation of unstably repressed rings was blocked by overexpression of silencing proteins Sir3p and Sir4p, and chromatin immunoprecipitation studies showed that overexpressed Sir3p was incorporated into silent chromatin. Importantly, the protein was incorporated even when expressed outside of S phase, during G(1) arrest. That silencing factors can associate with and stabilize preassembled silent chromatin in non-S-phase cells demonstrates that heterochromatin in yeast is dynamic.
Indexed as
Identifiers
10691737PMC316382W1915481228What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.