ArticleJournal of the American Society for Mass Spectrometry2000
Interpretation of matrix-assisted laser desorption/ionization postsource decay spectra of charge-derivatized peptides: some examples of tris[(2,4,6-trimethoxyphenyl) phosphonium]-tagged proteolytic digestion products of phosphoenolpyruvate carboxykinase.
Article in Journal of the American Society for Mass Spectrometry, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Photodissociation of charge tagged peptides.Journal of the American Society for Mass Spectrometry · 2012Article
- Tunable charge tags for electron-based methods of peptide sequencing: design and applications.Journal of the American Society for Mass Spectrometry · 2012Article
- Electron-capture and -transfer dissociation of peptides tagged with tunable fixed-charge groups: structures and dissociation energetics.Journal of the American Society for Mass Spectrometry · 2011Article
- Inter- and intra-molecular migration of peptide amide hydrogens during electrospray ionization.Journal of the American Society for Mass Spectrometry · 2001Article
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Authors and funding
2 authors.
Funding
Abstract
The fragmentation of peptides, to which a positive charge is attached at the N-terminus, was studied by matrix-assisted laser desorption/ionization postsource decay mass spectrometry. In these experiments, the tris[(2,4,6-trimethoxyphenyl)phosphonium] acetyl group is covalently attached. The main advantage of this modification is that the resulting spectra are simplified and the fragment ions observed consist predominantly of a(n)-type ions. We report the results for charge-derivatized peptides formed following enzymatic digestion of phosphoenolpyruvate carboxykinase. Specific fragmentation of bonds within aspargine and threonine residues are observed and are discussed. The understanding of the mechanistic aspects of the fragmentation process is essential to formulate a simple and straightforward mass spectrometric strategy for peptide sequencing using these charged derivatives.
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Registered trials
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