ArticleProceedings of the National Academy of Sciences of the United States of America2000
Isolation of peptide aptamers that inhibit intracellular processes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 72 citations in OpenAlex.
- Peptide Aptamer PA3 Attenuates the Viability ofFrontiers in microbiology · 2022Article
- Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library.Cell chemical biology · 2021Article
- Programmable biomaterials for dynamic and responsive drug delivery.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.BMC cell biology · 2010Article
- The eleven-year switch of peptide aptamers.Journal of biology · 2008Review
- Synthesis and selection of de novo proteins that bind and impede cellular functions of an essential mycobacterial protein.Applied and environmental microbiology · 2007Article
- Intracellular expression of Peptide fusions for demonstration of protein essentiality in bacteria.Antimicrobial agents and chemotherapy · 2003Article
- Peptide-mediated broad-spectrum plant resistance to tospoviruses.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Genetic selection for modulators of a retinoic-acid-responsive reporter in human cells.Genetics · 2003Article
- TEM-1 beta-lactamase as a scaffold for protein recognition and assay.Protein science : a publication of the Protein Society · 2002Article
- Targeted modification and transportation of cellular proteins.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Chemical genetics: ligand-based discovery of gene function.Nature reviews. Genetics · 2000Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a method for isolation of random peptides that inhibit intracellular processes in bacteria. A library of random peptides expressed as fusions to Escherichia coli thioredoxin (aptamers) were expressed under the tight control of the arabinose-inducible P(BAD) promoter. A selection was applied to the library to isolate aptamers that interfered with the activity of thymidylate synthase (ThyA) in vivo. Expression of an aptamer isolated by this method resulted in a ThyA(-) phenotype that was suppressed by simultaneous overexpression of ThyA. Two-hybrid analysis showed that this aptamer is likely to interact with ThyA in vivo. The library also was screened for aptamers that inhibited growth of bacteria expressing them, and five such aptamers were characterized. Four aptamers were bacteriostatic when expressed, whereas one showed a bactericidal effect. Introduction of translational stop codons into various aptamers blocked their activity, suggesting that their biological effects were likely to be due to protein aptamer rather than RNA. Combinatorial aptamers provide a new genetic and biochemical tool for identifying targets for antibacterial drug development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.