ArticleMolecular and cellular biology2000
Normal spermatogenesis in mice lacking the testis-specific linker histone H1t.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 133 citations in OpenAlex.
- Epigenetic regulation by DNA methylation, histone modifications and chromatin remodeling complexes in controlling spermatogenesis and their dysfunction with male infertility.Cellular and molecular life sciences : CMLS · 2025Review
- Post-Translation Modifications and Mutations of Human Linker Histone Subtypes: Their Manifestation in Disease.International journal of molecular sciences · 2023Review
- A rapidly evolving single copy histone H1 variant is associated with male fertility in a parasitoid wasp.Frontiers in cell and developmental biology · 2023Article
- The Art of Packaging the Sperm Genome: Molecular and Structural Basis of the Histone-To-Protamine Exchange.Frontiers in endocrinology · 2022Review
- Regulation of meiotic progression by Sertoli-cell androgen signaling.Molecular biology of the cell · 2020Article
- Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes.Epigenetics & chromatin · 2020Article
- Epigenetic changes in mammalian gametes throughout their lifetime: the four seasons metaphor.Chromosoma · 2019Review
- A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.Biochemistry · 2019Article
- Article
- Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements.Epigenetics & chromatin · 2018Article
- Linker Histone in Diseases.International journal of biological sciences · 2017Review
- Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X.Molecular and cellular biology · 2016Article
- Epigenetic regulation of the histone-to-protamine transition during spermiogenesis.Reproduction (Cambridge, England) · 2016Review
- Linker histone variant H1T targets rDNA repeats.Epigenetics · 2016Article
- Role of H1 linker histones in mammalian development and stem cell differentiation.Biochimica et biophysica acta · 2016Review
- Germline-specific H1 variants: the "sexy" linker histones.Chromosoma · 2016Review
- Local compartment changes and regulatory landscape alterations in histone H1-depleted cells.Genome biology · 2015Article
- What is the role of histone H1 heterogeneity? A functional model emerges from a 50 year mystery.AIMS biophysics · 2015Article
- Chromatin features and the epigenetic regulation of pluripotency states in ESCs.Development (Cambridge, England) · 2014Review
- Testicular morphology of male rats exposed to Phaleria macrocarpa (Mahkota dewa) aqueous extract.Iranian journal of basic medical sciences · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
H1 histones bind to linker DNA and nucleosome core particles and facilitate the folding of chromatin into a more compact structure. Mammals contain seven nonallelic subtypes of H1, including testis-specific subtype H1t, which varies considerably in primary sequence from the other H1 subtypes. H1t is found only in pachytene spermatocytes and early, haploid spermatids, constituting as much as 55% of the linker histone associated with chromatin in these cell types. To investigate the role of H1t in spermatogenesis, we disrupted the H1t gene by homologous recombination in mouse embryonic stem cells. Mice homozygous for the mutation and completely lacking H1t protein in their germ cells were fertile and showed no detectable defect in spermatogenesis. Chromatin from H1t-deficient germ cells had a normal ratio of H1 to nucleosomes, indicating that other H1 subtypes are deposited in chromatin in place of H1t and presumably compensate for most or all H1t functions. The results indicate that despite the unique primary structure and regulated synthesis of H1t, it is not essential for proper development of mature, functional sperm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.