ArticleRNA (New York, N.Y.)2000
A tertiary interaction detected in a human U2-U6 snRNA complex assembled in vitro resembles a genetically proven interaction in yeast.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Role of small nuclear RNAs in eukaryotic gene expression.Essays in biochemistry · 2013Review
- Structure of the yeast U2/U6 snRNA complex.RNA (New York, N.Y.) · 2012Article
- Metal binding and substrate positioning by evolutionarily invariant U6 sequences in catalytically active protein-free snRNAs.RNA (New York, N.Y.) · 2010Article
- Transposable elements in disease-associated cryptic exons.Human genetics · 2010Article
- A structural analysis of the group II intron active site and implications for the spliceosome.RNA (New York, N.Y.) · 2010Article
- Protein-free small nuclear RNAs catalyze a two-step splicing reaction.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
- The use of simple model systems to study spliceosomal catalysis.RNA (New York, N.Y.) · 2009Article
- Spliceosomal snRNAs in the unicellular eukaryote Trichomonas vaginalis are structurally conserved but lack a 5'-cap structure.RNA (New York, N.Y.) · 2008Article
- Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.RNA (New York, N.Y.) · 2007Article
- Aberrant 5' splice sites in human disease genes: mutation pattern, nucleotide structure and comparison of computational tools that predict their utilization.Nucleic acids research · 2007Article
- Use of a novel Förster resonance energy transfer method to identify locations of site-bound metal ions in the U2-U6 snRNA complex.Nucleic acids research · 2007Article
- Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.The EMBO journal · 2006Article
- Free energy landscapes of RNA/RNA complexes: with applications to snRNA complexes in spliceosomes.Journal of molecular biology · 2006Article
- New tertiary constraints between the RNA components of active yeast spliceosomes: a photo-crosslinking study.RNA (New York, N.Y.) · 2004Article
- Characterization of the catalytic activity of U2 and U6 snRNAs.RNA (New York, N.Y.) · 2003Article
- Characterization of U4 and U6 interactions with the 5' splice site using a S. cerevisiae in vitro trans-splicing system.Genes & development · 2001Article
- Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.Nucleic acids research · 2001Article
- Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome.Nature · 2000Article
- Nuclease protection of RNAs containing site-specific labels: a rapid method for mapping RNA-protein interactions.RNA (New York, N.Y.) · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
U2 and U6 small nuclear RNAs are thought to play critical roles in pre-mRNA splicing catalysis. Genetic evidence suggests they form an extensively base-paired structure within the spliceosome that is required for catalysis. Especially in light of significant similarities with group II self-splicing introns, we wished to investigate whether the purified RNAs might by themselves be able to form a complex similar to that which appears to exist in the spliceosome. To this end, we synthesized and purified large segments of human U2 and U6 snRNAs. Upon annealing, the two RNAs efficiently formed a stable and apparently extensively base-paired (Tm = 50-60 degrees C in the presence of 20 mM Mg2+) complex. To investigate possible tertiary interactions, we subjected the annealed complex to UV irradiation, and two crosslinked species were identified and characterized. The major one links the second G in the highly conserved and critical ACAGAGA sequence in U6 with an A in U2 just 5' to U2-U6 helix Ia and opposite the invariant AGC in U6. Remarkably, this crosslink indicates a tertiary interaction essentially identical to one detected previously by genetic covariation in yeast. Together our results suggest that purified U2 and U6 snRNAs can anneal and fold to form a structure resembling that likely to exist in the catalytically active spliceosome.
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