ArticleRNA (New York, N.Y.)2000
Mg2+-independent hairpin ribozyme catalysis in hydrated RNA films.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis.International journal of molecular sciences · 2024Article
- Research Advances on the Stability of mRNA Vaccines.Viruses · 2023Review
- Simple and Economical Extraction of Viral RNA and Storage at Ambient Temperature.Microbiology spectrum · 2022Article
- Nucleic Acid Catalysis under Potential Prebiotic Conditions.Chemistry, an Asian journal · 2020Review
- An efficient method for long-term room temperature storage of RNA.European journal of human genetics : EJHG · 2014Article
- Cobalt(III)hexaammine-dependent photocrosslinks in the hairpin ribozyme.Journal of inorganic biochemistry · 2014Article
- A comparison of vanadate to a 2'-5' linkage at the active site of a small ribozyme suggests a role for water in transition-state stabilization.RNA (New York, N.Y.) · 2007Article
- Ligation of the hairpin ribozyme in cis induced by freezing and dehydration.RNA (New York, N.Y.) · 2006Article
- Evidence for ditopic coordination of phosphate diesters to [Mg(15-crown-5)]2+. Implications for magnesium biocoordination chemistry.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2004Article
- Importance in catalysis of a magnesium ion with very low affinity for a hammerhead ribozyme.Nucleic acids research · 2004Article
- Ligation activity of fragmented ribozymes in frozen solution: implications for the RNA world.Nucleic acids research · 2004Article
- Recent advances in the elucidation of the mechanisms of action of ribozymes.Nucleic acids research · 2001Review
- The hammerhead cleavage reaction in monovalent cations.RNA (New York, N.Y.) · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hairpin ribozyme catalyzes RNA cleavage in partially hydrated RNA films in the absence of added divalent cations. This reaction exhibits the characteristics associated with the RNA cleavage reaction observed under standard conditions in solution. Catalysis is a site-specific intramolecular transesterification reaction, requires the 2'-hydroxyl group of substrate nucleotide A(-1), and generates 2',3'-cyclic phosphate and 5'-hydroxyl termini. Mutations in both ribozyme and substrate abolish catalysis in hydrated films. The reaction is accelerated by cations that may enhance binding, conformational stability, and catalytic activity, and is inhibited by Tb3+. The reaction has an apparent temperature optimum of 4 degrees C. At this temperature, cleavage is slow (k(obs): 2 d(-1)) and progressive, with accumulation of cleavage products to an extent of 40%. The use of synthetic RNAs, chelators, and analysis of all reaction components by inductively coupled plasma-optical spectrophotometry (ICPOES) effectively rules out the possibility of contaminating divalent metals in the reactions. Catalysis is minimal under conditions of extreme dehydration, indicating that the reaction requires hydration of RNA by atmospheric water. Our results provide a further caution for those studying the biochemical activity of ribozymes in vitro and in cells, as unanticipated catalysis could occur during RNA manipulation and lead to misinterpretation of data.
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