ArticleRNA (New York, N.Y.)2000
A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- SF3B1 phosphorylation is an evolutionarily conserved step in spliceosome activation carried out by the divergent, OTS964-insensitive kinase CRK9 in trypanosomes.Nucleic acids research · 2026Article
- Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa).RNA (New York, N.Y.) · 2025Article
- Comprehensive analysis of the Kinetoplastea intron landscape reveals a novel intron-containing gene and the first exclusively trans-splicing eukaryote.BMC biology · 2024Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Article
- Current Status of Regulatory Non-Coding RNAs Research in the Tritryp.Non-coding RNA · 2022Review
- LncRNAs Are Differentially Expressed between Wildtype and Cell Line Strains of African Trypanosomes.Non-coding RNA · 2022Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes.Nucleic acids research · 2021Article
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation iniScience · 2020Article
- Evolution of metabolic capabilities and molecular features of diplonemids, kinetoplastids, and euglenids.BMC biology · 2020Article
- Analysis of mRNA processing at whole transcriptome level, transcriptomic profile and genome sequence refinement of Trypanosoma cruzi.Scientific reports · 2019Article
- African trypanosomes.Parasites & vectors · 2019Article
- Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation.Scientific reports · 2019Article
- Transcriptome, proteome and draft genome of Euglena gracilis.BMC biology · 2019Article
- Trypanosomes can initiate nuclear export co-transcriptionally.Nucleic acids research · 2019Article
- Order of removal of conventional and nonconventional introns from nuclear transcripts of Euglena gracilis.PLoS genetics · 2018Article
- Clinical and veterinary trypanocidal benzoxaboroles target CPSF3.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
12 more citing papers are in PubMed but not listed here.
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14 authors.
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Abstract
It has been known for almost a decade and a half that in trypanosomes all mRNAs are trans-spliced by addition to the 5' end of the spliced leader (SL) sequence. During the same time period the conviction developed that classical cis-splicing introns are not present in the trypanosome genome and that the trypanosome gene arrangement is highly compact with small intergenic regions separating one gene from the next. We have now discovered that these tenets are no longer true. Poly(A) polymerase (PAP) genes in Trypanosoma brucei and Trypanosoma cruzi are split by intervening sequences of 653 and 302 nt, respectively. The intervening sequences occur at identical positions in both organisms and obey the GT/AG rule of cis-splicing introns. PAP mRNAs are trans-spliced at the very 5' end as well as internally at the 3' splice site of the intervening sequence. Interestingly, 11 nucleotide positions past the actual 5' splice site are conserved between the T. bruceiand T. cruzi introns. Point mutations in these conserved positions, as well as in the AG dinucleotide of the 3' splice site, abolish intron removal in vivo. Our results, together with the recent discovery of cis-splicing introns in Euglena gracilis, suggest that both trans- and cis-splicing are ancient acquisitions of the eukaryotic cell.
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