ArticleNucleic acids research2000
A developmentally regulated deletion element with long terminal repeats has cis-acting sequences in the flanking DNA.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Natural history of eukaryotic DNA viruses with double jelly-roll major capsid proteins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Natural history of eukaryotic DNA viruses with double jelly-roll major capsid proteins.bioRxiv : the preprint server for biology · 2024Article
- Setting boundaries for genome-wide heterochromatic DNA deletions through flanking inverted repeats in Tetrahymena thermophila.Nucleic acids research · 2019Article
- Structure of the germline genome ofeLife · 2016Article
- A Parallel G Quadruplex-Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila.PLoS genetics · 2016Article
- The taming of the shrew: Regulation of a catalytically active domesticated transposase.Mobile genetic elements · 2014Article
- Keeping the soma free of transposons: programmed DNA elimination in ciliates.The Journal of biological chemistry · 2011Review
- RNA-Mediated Epigenetic Programming of Genome Rearrangements.Annual review of genomics and human genetics · 2011Review
- Subtraction by addition: domesticated transposases in programmed DNA elimination.Genes & development · 2009Article
- The germ line limited M element of Tetrahymena is targeted for elimination from the somatic genome by a homology-dependent mechanism.Nucleic acids research · 2006Article
- Germ line transcripts are processed by a Dicer-like protein that is essential for developmentally programmed genome rearrangements of Tetrahymena thermophila.Molecular and cellular biology · 2005Article
- Role of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.Eukaryotic cell · 2004Article
- Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.Eukaryotic cell · 2003Article
- A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophila.Nucleic acids research · 2002Article
- Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila.The EMBO journal · 2001Article
- Cis-acting requirements in flanking DNA for the programmed elimination of mse2.9: a common mechanism for deletion of internal eliminated sequences from the developing macronucleus of Tetrahymena thermophila.Nucleic acids research · 2001Article
- A family of developmentally excised DNA elements in Tetrahymena is under selective pressure to maintain an open reading frame encoding an integrase-like protein.Nucleic acids research · 2000Article
- Developmentally regulated excision of a 28-base-pair sequence from the Paramecium genome requires flanking DNA.Molecular and cellular biology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Approximately 6000 specific DNA deletion events occur during development of the somatic macro-nucleus of the ciliate Tetrahymena. The eliminated Tlr1 element is 13 kb or more in length and has an 825 bp inverted repeat near the rearrangement junctions. A functional analysis of the cis -acting sequences required for Tlr1 rearrangement was performed. A construct consisting of the entire inverted repeat and several hundred base pairs of flanking DNA on each side was rearranged accurately in vivo and displayed junctional variability similar to the chromosomal Tlr1 rearrangement. Thus, 11 kb or more of internal element DNA is not required in cis for DNA rearrangement. A second construct with only 51 bp of Tetra-hymena DNA flanking the right junction underwent aberrant rearrangement. Thus, a signal for determination of the Tlr1 junction is located in the flanking DNA, 51 bp or more from the right junction. Within the Tlr1 inverted repeat are 19 bp tandem repeats. A construct with the 19mer repeat region deleted from the right half of the inverted repeat utilized normal rearrangement junctions. Thus, despite its transposon-like structure, Tlr1 is similar to other DNA rearrangements in Tetrahymena in possessing cis -acting sequences outside the deleted DNA.
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