ArticleNucleic acids research2000
Interaction of the yeast DExH-box RNA helicase prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Genome-wide modulation of alternative splicing by a predicted alpha helix in U2AF2.Nucleic acids research · 2025Article
- psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation.Nature communications · 2021Article
- Structure of the yeast spliceosomal postcatalytic P complex.Science (New York, N.Y.) · 2017Article
- Yeast Prp2 liberates the 5' splice site and the branch site adenosine for catalysis of pre-mRNA splicing.RNA (New York, N.Y.) · 2017Article
- Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing.Journal of biomedical science · 2015Review
- Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.PLoS genetics · 2015Article
- Proteins associated with SF3a60 in T. brucei.PloS one · 2014Article
- Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.RNA (New York, N.Y.) · 2013Article
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- Prp22 and spliceosome components regulate chromatin dynamics in germ-line polyploid cells.PloS one · 2013Article
- RNA helicases in splicing.RNA biology · 2013Review
- Functional roles of protein splicing factors.Bioscience reports · 2012Review
- Staying on message: ensuring fidelity in pre-mRNA splicing.Trends in biochemical sciences · 2012Review
- Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway.Molecular and cellular biology · 2011Article
- Spliceosome discards intermediates via the DEAH box ATPase Prp43p.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- PRP-17 and the pre-mRNA splicing pathway are preferentially required for the proliferation versus meiotic development decision and germline sex determination in Caenorhabditis elegans.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing.RNA (New York, N.Y.) · 2010Article
- A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release.Molecular cell · 2008Article
- "Nought may endure but mutability": spliceosome dynamics and the regulation of splicing.Molecular cell · 2008Review
- Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.RNA (New York, N.Y.) · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Using site-specific incorporation of the photo-chemical cross-linking reagent 4-thiouridine, we demonstrate the previously unknown association of two proteins with yeast 3' splice sites. One of these is an unidentified approximately 122 kDa protein that cross-links to 3' splice sites during formation of the pre--spliceosome. The other factor is the DExH-box RNA helicase, Prp22p. With substrates functional in the second step of splicing, only very weak cross-linking of Prp22p to intron sequences at the 3' splice site is observed. In contrast, substrates blocked at the second step exhibit strong cross-linking of Prp22 to intron sequences at the 3' splice site, but not to adjacent exon sequences. In vitro reconstitution experiments also show that the association of Prp22p with intron sequences at the 3' splice site is dependent on Prp16p and does not persist when release of mature mRNA from the spliceosome is blocked. Taken together, these results suggest that the 3' splice site of yeast introns is contacted much earlier than previously envisioned by a protein of approximately 120 kDa, and that a transient association of Prp22p with the 3' splice site occurs between the first and second catalytic steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.