ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
beta subunits modulate alternatively spliced, large conductance, calcium-activated potassium channels of avian hair cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- High-frequency electrical tuning and linear filter properties of Knollenorgan electroreceptors of mormyrid electric fish.The Journal of physiology · 2025Article
- Alternative splicing in shaping the molecular landscape of the cochlea.Frontiers in cell and developmental biology · 2023Review
- Calcium-induced calcium release in proximity to hair cell BK channels revealed by PKA activation.Physiological reports · 2020Article
- Effects of aerobic exercise training on large-conductance Ca(2+)-activated K (+) channels in rat cerebral artery smooth muscle cells.European journal of applied physiology · 2013Article
- CDK5 interacts with Slo and affects its surface expression and kinetics through direct phosphorylation.American journal of physiology. Cell physiology · 2012Article
- Saccular-specific hair cell addition correlates with reproductive state-dependent changes in the auditory saccular sensitivity of a vocal fish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Shaping of action potentials by type I and type II large-conductance Ca²+-activated K+ channels.Neuroscience · 2011Article
- Gene expression gradients along the tonotopic axis of the chicken auditory epithelium.Journal of the Association for Research in Otolaryngology : JARO · 2011Article
- β4-subunit increases Slo responsiveness to physiological Ca2+ concentrations and together with β1 reduces surface expression of Slo in hair cells.American journal of physiology. Cell physiology · 2011Article
- Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat.Pflugers Archiv : European journal of physiology · 2011Article
- Cloning and distribution of Ca2+-activated K+ channels in lobster Panulirus interruptus.Neuroscience · 2010Article
- Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development.The Journal of comparative neurology · 2010Article
- Highly specific alternative splicing of transcripts encoding BK channels in the chicken's cochlea is a minor determinant of the tonotopic gradient.Molecular and cellular biology · 2010Article
- Developmental expression of BK channels in chick cochlear hair cells.BMC developmental biology · 2009Article
- Tamoxifen inhibits BK channels in chick cochlea without alterations in voltage-dependent activation.American journal of physiology. Cell physiology · 2009Article
- Voltage-activated ion channels and Ca(2+)-induced Ca (2+) release shape Ca (2+) signaling in Merkel cells.Pflugers Archiv : European journal of physiology · 2008Article
- Alternatively spliced C-terminal domains regulate the surface expression of large conductance calcium-activated potassium channels.Neuroscience · 2007Article
- Tonotopic gradients of membrane and synaptic properties for neurons of the chicken nucleus magnocellularis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Effects of intracellular stores and extracellular Ca(2+) on Ca(2+)-activated K(+) currents in mature mouse inner hair cells.The Journal of physiology · 2004Article
- Association of beta-catenin with the alpha-subunit of neuronal large-conductance Ca2+-activated K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Electrical tuning confers frequency selectivity onto sensory hair cells in the auditory periphery of frogs, turtles, and chicks. The resonant frequency is determined in large part by the number and kinetics of large conductance, calcium-activated potassium (BK) channels. BK channels in hair cells are encoded by the alternatively spliced slo gene and may include an accessory beta subunit. Here we examine the origins of kinetic variability among BK channels by heterologous expression of avian cochlear slo cDNAs. Four alternatively spliced forms of the slo-alpha gene from chick hair cells were co-expressed with accessory beta subunits (from quail cochlea) by transient transfection of human embryonic kidney 293 cells. Addition of the beta subunit increased steady-state calcium affinity, raised the Hill coefficient for calcium binding, and slowed channel deactivation rates, resulting in eight functionally distinct channels. For example, a naturally occurring splice variant containing three additional exons deactivated 20-fold more slowly when combined with beta. Deactivation kinetics were used to predict tuning frequencies and thus tonotopic location if hair cells were endowed with each of the expressed channels. All beta-containing channels were predicted to lie within the apical (low-frequency) 30% of the epithelium, consistent with previous in situ hybridization studies. Individual slo-alpha exons would be found anywhere within the apical 70%, depending on the presence of beta, and other alternative exons. Alternative splicing of the slo-alpha channel message provides intrinsic variability in gating kinetics that is expanded to a wider range of tuning by modulation with beta subunits.
Indexed as
Identifiers
10684869PMC6772940W2117171932What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.