ArticleJournal of virology2000
Functional characterization of the human immunodeficiency virus type 1 genome by genetic footprinting.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.Molecular genetics and genomics : MGG · 2016Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- High-resolution functional profiling of the norovirus genome.Journal of virology · 2012Article
- cis-Acting determinants of 7SL RNA packaging by HIV-1.Journal of virology · 2012Article
- High-resolution functional profiling of a gammaherpesvirus RTA locus in the context of the viral genome.Journal of virology · 2009Article
- Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice.PloS one · 2009Article
- Bacteriophage Mu integration in yeast and mammalian genomes.Nucleic acids research · 2008Article
- High-resolution functional profiling of hepatitis C virus genome.PLoS pathogens · 2008Article
- High-precision mapping of protein protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform.Nucleic acids research · 2007Article
- Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins.Journal of virology · 2006Article
- Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.Journal of virology · 2006Article
- Selection of novel vesicular stomatitis virus glycoprotein variants from a peptide insertion library for enhanced purification of retroviral and lentiviral vectors.Journal of virology · 2006Article
- A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach.Nucleic acids research · 2005Article
- Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replication.Journal of virology · 2004Article
- Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes.Journal of virology · 2004Article
- Functional characterization of a portion of the Moloney murine leukemia virus gag gene by genetic footprinting.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A direct transposon insertion tool for modification and functional analysis of viral genomes.Journal of virology · 2003Article
- Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.Genome research · 2002Article
- Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu.Applied and environmental microbiology · 2002Article
- Comprehensive mutational analysis of the Moloney murine leukemia virus envelope protein.Journal of virology · 2001Article
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
We present a detailed and quantitative analysis of the functional characteristics of the 1,000-nucleotide segment at the 5' end of the human immunodeficiency virus type 1 (HIV-1) RNA genome. This segment of the viral genome contains several important cis-acting sequences, including the TAR, polyadenylation, viral att site, minus-strand primer-binding site, and 5' splice donor sequences, as well as coding sequences for the matrix protein and the N-terminal half of the capsid protein. The genetic footprinting technique was used to determine quantitatively the abilities of 134 independent insertion mutations to (i) make stable viral RNA, (ii) assemble and release viral RNA-containing viral particles, and (iii) enter host cells, complete reverse transcription, enter the nuclei of host cells, and generate proviruses in the host genome by integration. All of the mutants were constructed and analyzed en masse, greatly decreasing the labor typically involved in mutagenesis studies. The results confirmed the presence of several previously known functional features in this region of the HIV genome and provided evidence for several novel features, including newly identified cis-acting sequences that appeared to contribute to (i) the formation of stable viral transcripts, (ii) viral RNA packaging, and (iii) an early step in viral replication. The results also pointed to an unanticipated trans-acting role for the N-terminal portion of matrix in the formation of stable viral RNA transcripts. Finally, in contrast to previous reports, the results of this study suggested that detrimental mutations in the matrix and capsid proteins principally interfered with viral assembly.
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