ArticleJournal of virology2000
Replication of lengthened Moloney murine leukemia virus genomes is impaired at multiple stages.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Collapsing retroviruses for efficient delivery of viro-toxic cargoes.bioRxiv : the preprint server for biology · 2026Article
- Article
- The impact of lentiviral vector genome size and producer cell genomic to gag-pol mRNA ratios on packaging efficiency and titre.Molecular therapy. Methods & clinical development · 2021Article
- Determinants of Moloney murine leukemia virus Gag-Pol and genomic RNA proportions.Journal of virology · 2014Article
- Dimeric RNA recognition regulates HIV-1 genome packaging.PLoS pathogens · 2013Article
- Influence of insulators on transgene expression from integrating and non-integrating lentiviral vectors.Genetic vaccines and therapy · 2011Article
- Retroviral RNA dimerization and packaging: the what, how, when, where, and why.PLoS pathogens · 2010Review
- Mechanism of reduction in titers from lentivirus vectors carrying large inserts in the 3'LTR.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- The remarkable frequency of human immunodeficiency virus type 1 genetic recombination.Microbiology and molecular biology reviews : MMBR · 2009Review
- Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors.BMC molecular biology · 2009Article
- Effects of identity minimization on Moloney murine leukemia virus template recognition and frequent tertiary template-directed insertions during nonhomologous recombination.Journal of virology · 2007Article
- Tissue- and tumor-specific targeting of murine leukemia virus-based replication-competent retroviral vectors.Journal of virology · 2006Article
- Retrovirus infection strongly enhances scrapie infectivity release in cell culture.The EMBO journal · 2006Article
- Nonrandom dimerization of murine leukemia virus genomic RNAs.Journal of virology · 2004Article
- Human immunodeficiency virus type 1 transductive recombination can occur frequently and in proportion to polyadenylation signal readthrough.Journal of virology · 2004Article
- Human immunodeficiency virus type 1 genetic recombination is more frequent than that of Moloney murine leukemia virus despite similar template switching rates.Journal of virology · 2003Article
- Efficient human immunodeficiency virus replication requires a fine-tuned level of transcription.Journal of virology · 2002Article
- RNase H activity is required for high-frequency repeat deletion during Moloney murine leukemia virus replication.Journal of virology · 2002Article
- Effects of limiting homology at the site of intermolecular recombinogenic template switching during Moloney murine leukemia virus replication.Journal of virology · 2001Article
- Genomic stability of murine leukemia viruses containing insertions at the Env-3' untranslated region boundary.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
It has been assumed that RNA packaging constraints limit the size of retroviral genomes. This notion of a retroviral "headful" was tested by examining the ability of Moloney murine leukemia virus genomes lengthened by 4, 8, or 11 kb to participate in a single replication cycle. Overall, replication of these lengthened genomes was 5- to 10-fold less efficient than that of native-length genomes. When RNA expression and virion formation, RNA packaging, and early stages of replication were compared, long genomes were found to complete each step less efficiently than did normal-length genomes. To test whether short RNAs might facilitate the packaging of lengthy RNAs by heterodimerization, some experiments involved coexpression of a short packageable RNA. However, enhancement of neither long vector RNA packaging nor long vector DNA synthesis was observed in the presence of the short RNA. Most of the proviruses templated by 12 and 16 kb vectors appeared to be full length. Most products of a 19. 2-kb vector contained deletions, but some integrated proviruses were around twice the native genome length. These results demonstrate that lengthy retroviral genomes can be packaged and that genome length is not strictly limited at any individual replication step. These observations also suggest that the lengthy read-through RNAs postulated to be intermediates in retroviral transduction can be packaged directly without further processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.