Evidence map›Paper›PMID 10684267›Full record

ArticleJournal of virology2000

Feline immunodeficiency virus Vif localizes to the nucleus.

U Chatterji, C K Grant, J H Elder

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

U ChatterjiDepartment of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
C K Grant
J H Elder
Scripps Research Institute · US

Funding

MOLECULAR CHARACTERIZATION OF FIVR01AI025825 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI ELDER, JOHN H · 1987 to 2015
$6.0M
Substrate/Inhibitor Specificity of FIV and HIV PRsR01AI040882 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI ELDER, JOHN H · 1997 to 2006
$1.6M
NIAID NIH HHS R01 AI025825NIAID NIH HHS R01 AI040882NIAID NIH HHS R01 AI25825NIAID NIH HHS R01 AI40882
6 · The paper itself

Abstract

Monoclonal antibodies prepared against recombinant Vif derived from the 34TF10 strain of feline immunodeficiency virus (FIV) were used to assess the expression and localization of Vif in virus-infected cells. Analyses by Western blotting and by immunoprecipitation from cells infected with FIV-34TF10 revealed the presence of a single 29-kDa species specific for virus-infected cells. Confirmation of antibody specificity was also performed by specific immunoprecipitation of in vitro-transcribed and -translated recombinant Vif. Localization experiments were also performed on virus-infected cells, using different fixation procedures. Results for methanol fixation protocols similar to those reported for localization of human immunodeficiency virus (HIV) Vif showed a predominant cytoplasmic localization for FIV Vif, very similar to localization of HIV type 1 Vif and virtually identical to the localization observed for the Gag antigens of the virus. However, with milder fixation procedures that used 2% formaldehyde at 4 degrees C, FIV Vif was strongly evident in the nucleus. The localization was distinct from the nuclear localization noted with Rev and did not involve the nucleolus. Attempts to show colocalization or coprecipitation of Vif with Gag antigens were unsuccessful. In addition, Vif was not detected in purified FIV virions. The results are consistent with the notion that the primary role of Vif in virus infection initiates in the nucleus.

Indexed as

Amino Acid SequenceAnimalsBase SequenceCatsCell LineCell NucleusCytoplasmFemaleGene Products, gagGene Products, vifImmunodeficiency Virus, FelineMiceMice, Inbred BALB CMolecular Sequence DataProtein PrecursorsGene Products, gagGene Products, vifProtein Precursors

Identifiers

PMID10684267
PMCPMC111741
OpenAlexW2100010017

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.