ArticleThe Journal of cell biology2000
A transmembrane segment determines the steady-state localization of an ion-transporting adenosine triphosphatase.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 84 citations in OpenAlex.
- PolarProtPred: predicting apical and basolateral localization of transmembrane proteins using putative short linear motifs and deep learning.Bioinformatics (Oxford, England) · 2021Article
- Regulation of Transporters and Channels by Membrane-Trafficking Complexes in Epithelial Cells.Cold Spring Harbor perspectives in biology · 2017Review
- The Apical Localization of NaFrontiers in physiology · 2016Article
- Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin.Molecular biology of the cell · 2015Article
- Trafficking to the apical and basolateral membranes in polarized epithelial cells.Journal of the American Society of Nephrology : JASN · 2014Review
- Pancreatic bicarbonate secretion involves two proton pumps.The Journal of biological chemistry · 2011Article
- Protein phosphatase 2A interacts with the Na,K-ATPase and modulates its trafficking by inhibition of its association with arrestin.PloS one · 2011Article
- Tyrosine-dependent basolateral targeting of human connexin43-eYFP in Madin-Darby canine kidney cells can be disrupted by the oculodentodigital dysplasia mutation L90V.The FEBS journal · 2009Article
- Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells.The Journal of cell biology · 2009Article
- Exon loss accounts for differential sorting of Na-K-Cl cotransporters in polarized epithelial cells.Molecular biology of the cell · 2008Article
- Transmembrane domain-dependent partitioning of membrane proteins within the endoplasmic reticulum.The Journal of cell biology · 2008Article
- Sodium pump localization in epithelia.Journal of bioenergetics and biomembranes · 2007Review
- Arrestins and spinophilin competitively regulate Na+,K+-ATPase trafficking through association with a large cytoplasmic loop of the Na+,K+-ATPase.Molecular biology of the cell · 2007Article
- MAL decreases the internalization of the aquaporin-2 water channel.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells.Molecular biology of the cell · 2005Article
- Cholesterol-induced protein sorting: an analysis of energetic feasibility.Biophysical journal · 2003Article
- Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The H,K-adenosine triphosphatase (ATPase) of gastric parietal cells is targeted to a regulated membrane compartment that fuses with the apical plasma membrane in response to secretagogue stimulation. Previous work has demonstrated that the alpha subunit of the H, K-ATPase encodes localization information responsible for this pump's apical distribution, whereas the beta subunit carries the signal responsible for the cessation of acid secretion through the retrieval of the pump from the surface to the regulated intracellular compartment. By analyzing the sorting behaviors of a number of chimeric pumps composed of complementary portions of the H, K-ATPase alpha subunit and the highly homologous Na,K-ATPase alpha subunit, we have identified a portion of the gastric H,K-ATPase, which is sufficient to redirect the normally basolateral Na,K-ATPase to the apical surface in transfected epithelial cells. This motif resides within the fourth of the H,K-ATPase alpha subunit's ten predicted transmembrane domains. Although interactions with glycosphingolipid-rich membrane domains have been proposed to play an important role in the targeting of several apical membrane proteins, the apically located chimeras are not found in detergent-insoluble complexes, which are typically enriched in glycosphingolipids. Furthermore, a chimera incorporating the Na, K-ATPase alpha subunit fourth transmembrane domain is apically targeted when both of its flanking sequences derive from H,K-ATPase sequence. These results provide the identification of a defined apical localization signal in a polytopic membrane transport protein, and suggest that this signal functions through conformational interactions between the fourth transmembrane spanning segment and its surrounding sequence domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.