ArticleThe Journal of cell biology2000
Live Salmonella recruits N-ethylmaleimide-sensitive fusion protein on phagosomal membrane and promotes fusion with early endosome.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
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- Endomembrane remodeling and dynamics inMicrobial cell (Graz, Austria) · 2022Review
- Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis.Cellular and molecular life sciences : CMLS · 2022Article
- A trafficome-wide RNAi screen reveals deployment of early and late secretory host proteins and the entire late endo-/lysosomal vesicle fusion machinery by intracellular Salmonella.PLoS pathogens · 2020Article
- Proteomic Analysis ofMolecular & cellular proteomics : MCP · 2020Article
- RNAseq Profiling of Leukocyte Populations in Zebrafish Larvae Reveals aFrontiers in immunology · 2019Article
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- Reticulon 3 regulates very low density lipoprotein secretion by controlling very low density lipoprotein transport vesicle biogenesis.Canadian journal of physiology and pharmacology · 2018Article
- Sequential actions of phosphatidylinositol phosphates regulate phagosome-lysosome fusion.Molecular biology of the cell · 2018Article
- Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494.PLoS pathogens · 2017Article
- Single GDP-dissociation Inhibitor Protein regulates endocytic and secretory pathways in Leishmania.Scientific reports · 2016Article
- Relocalization of junctional adhesion molecule A during inflammatory stimulation of brain endothelial cells.Molecular and cellular biology · 2012Article
- Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6.The Journal of biological chemistry · 2012Article
- Cell-free fusion of bacteria-containing phagosomes with endocytic compartments.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The identification of the SNARE complex required for the fusion of VLDL-transport vesicle with hepatic cis-Golgi.The Biochemical journal · 2010Article
- The HLA-DRalpha chain is modified by polyubiquitination.The Journal of biological chemistry · 2009Article
- Leishmania requires Rab7-mediated degradation of endocytosed hemoglobin for their growth.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Salmonella trafficking is defined by continuous dynamic interactions with the endolysosomal system.Traffic (Copenhagen, Denmark) · 2007Article
- IL-6 and IL-12 specifically regulate the expression of Rab5 and Rab7 via distinct signaling pathways.The EMBO journal · 2006Article
- Vesicle-associated membrane protein 7 is expressed in intestinal ER.Journal of cell science · 2006Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand intracellular trafficking modulations by live Salmonella, we investigated the characteristics of in vitro fusion between endosomes and phagosomes containing live (LSP) or dead Salmonella (DSP). We observed that fusion of both DSP and LSP were time, temperature and cytosol dependent. GTPgammaS and treatment of the phagosomes with Rab-GDI inhibited fusion, indicating involvement of Rab-GTPases. LSP were rich in rab5, alpha-SNAP, and NSF, while DSP mainly contained rab7. Fusion of endosomes with DSP was inhibited by ATP depletion, N-ethylmaleimide (NEM) treatment, and in NEM-sensitive factor (NSF)-depleted cytosol. In contrast, fusion of endosomes with LSP was not inhibited by ATP depletion or NEM treatment, and occurred in NSF-depleted cytosol. However, ATPgammaS inhibited both fusion events. Fusion of NEM-treated LSP with endosomes was abrogated in NSF- depleted cytosol and was restored by adding purified NSF, whereas no fusion occurred with NEM-treated DSP, indicating that NSF recruitment is dependent on continuous signals from live Salmonella. Binding of NSF with LSP required prior presence of rab5 on the phagosome. We have also shown that rab5 specifically binds with Sop E, a protein from Salmonella. Our results indicate that live Salmonella help binding of rab5 on the phagosomes, possibly activate the SNARE which leads to further recruitment of alpha-SNAP for subsequent binding with NSF to promote fusion of the LSP with early endosomes and inhibition of their transport to lysosomes.
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