ArticleThe Journal of cell biology2000
Induction of caveolae in the apical plasma membrane of Madin-Darby canine kidney cells.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 110 citations in OpenAlex.
- Langmuir Monolayers as Effective Models of Apical Epithelial Cell Membranes: Studies on the Effects of Phthalate Incorporation.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Lipid-anchored melanotransferrin mediates transferrin-independent iron uptake and ferritin storage in mammals.Cell death discovery · 2026Article
- Advances in development of antiviral strategies against respiratory syncytial virus.Acta pharmaceutica Sinica. B · 2025Review
- Article
- CHIMs are versatile cholesterol analogs mimicking and visualizing cholesterol behavior in lipid bilayers and cells.Communications biology · 2021Article
- Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells.Cell and tissue research · 2016Article
- Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells.American journal of physiology. Renal physiology · 2013Article
- Methods for the study of dopamine receptors within lipid rafts of kidney cells.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells.Experimental cell research · 2012Article
- Polarization and myelination in myelinating glia.ISRN neurology · 2012Article
- Receptor-like Molecules on Human Intestinal Epithelial Cells Interact with an Adhesion Factor from Lactobacillus reuteri.Bioscience of microbiota, food and health · 2012Article
- Article
- Sorting it out in endosomes: an emerging concept in renal epithelial cell transport regulation.Physiology (Bethesda, Md.) · 2010Review
- KIF5C, a kinesin motor involved in apical trafficking of MDCK cells.Cellular and molecular life sciences : CMLS · 2010Article
- CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport: relevance to pathophysiology of Dent's disease.American journal of physiology. Renal physiology · 2010Article
- Role of N-glycosylation in trafficking of apical membrane proteins in epithelia.American journal of physiology. Renal physiology · 2009Review
- Uptake of long chain fatty acids is regulated by dynamic interaction of FAT/CD36 with cholesterol/sphingolipid enriched microdomains (lipid rafts).BMC cell biology · 2008Article
- Hydrocortisone modulates cholera toxin endocytosis by regulating immature enterocyte plasma membrane phospholipids.Gastroenterology · 2008Article
- Clathrin-independent endocytosis: from nonexisting to an extreme degree of complexity.Histochemistry and cell biology · 2008Review
- Plasma membrane domain organization regulates EGFR signaling in tumor cells.The Journal of cell biology · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we have analyzed the behavior of antibody cross-linked raft-associated proteins on the surface of MDCK cells. We observed that cross-linking of membrane proteins gave different results depending on whether cross-linking occurred on the apical or basolateral plasma membrane. Whereas antibody cross-linking induced the formation of large clusters on the basolateral membrane, resembling those observed on the surface of fibroblasts (Harder, T., P. Scheiffele, P. Verkade, and K. Simons. 1998. J. Cell Biol. 929-942), only small ( approximately 100 nm) clusters formed on the apical plasma membrane. Cross-linked apical raft proteins e.g., GPI-anchored placental alkaline phosphatase (PLAP), influenza hemagglutinin, and gp114 coclustered and were internalized slowly ( approximately 10% after 60 min). Endocytosis occurred through surface invaginations that corresponded in size to caveolae and were labeled with caveolin-1 antibodies. Upon cholesterol depletion the internalization of PLAP was completely inhibited. In contrast, when a non-raft protein, the mutant LDL receptor LDLR-CT22, was cross-linked, it was excluded from the clusters of raft proteins and was rapidly internalized via clathrin-coated pits. Since caveolae are normally present on the basolateral membrane but lacking from the apical side, our data demonstrate that antibody cross-linking induced the formation of caveolae, which slowly internalized cross-linked clusters of raft-associated proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.