ArticleThe Journal of clinical investigation2000
Dynein light chain binding to a 3'-untranslated sequence mediates parathyroid hormone mRNA association with microtubules.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 38 citations in OpenAlex.
- Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation.FEBS letters · 2018Article
- NMR Characterization of Self-Association Domains Promoted by Interactions with LC8 Hub Protein.Computational and structural biotechnology journal · 2014Review
- Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons.Scientific reports · 2011Article
- Hijacking ZIP codes: posttanscriptional regulation of CCN2 by nucleophosmin.Journal of cell communication and signaling · 2009Article
- The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Cyclic AMP inhibits p38 activation via CREB-induced dynein light chain.Molecular and cellular biology · 2006Article
- Cytoplasmic dynein-dynactin complex is required for spermatid growth but not axoneme assembly in Drosophila.Molecular biology of the cell · 2004Article
- Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.The Biochemical journal · 2004Article
- PLAC-24 is a cytoplasmic dynein-binding protein that is recruited to sites of cell-cell contact.Molecular biology of the cell · 2002Article
- African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein.Journal of virology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The 3'-untranslated region (UTR) of mRNAs binds proteins that determine mRNA stability and localization. The 3'-UTR of parathyroid hormone (PTH) mRNA specifically binds cytoplasmic proteins. We screened an expression library for proteins that bind the PTH mRNA 3'-UTR, and the sequence of 1 clone was identical to that of the dynein light chain LC8, a component of the dynein complexes that translocate cytoplasmic components along microtubules. Recombinant LC8 binds PTH mRNA 3'-UTR, as shown by RNA electrophoretic mobility shift assay. We showed that PTH mRNA colocalizes with microtubules in the parathyroid gland, as well as with a purified microtubule preparation from calf brain, and that this association was mediated by LC8. To our knowledge, this is the first report of a dynein complex protein binding an mRNA. The dynein complex may be the motor that is responsible for transporting mRNAs to specific locations in the cytoplasm and for the consequent is asymmetric distribution of translated proteins in the cell.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.