ArticleBritish journal of pharmacology2000
Evidence that rat hepatocytes co-express functional P2Y1 and P2Y2 receptors.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Receptor-specific CaiScience · 2021Article
- Important roles of P2Y receptors in the inflammation and cancer of digestive system.Oncotarget · 2016Review
- Purinergic signalling in the liver in health and disease.Purinergic signalling · 2014Review
- Calcium signaling in the liver.Comprehensive Physiology · 2013Review
- Activated hepatic stellate cells upregulate transcription of ecto-5'-nucleotidase/CD73 via specific SP1 and SMAD promoter elements.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Calcium-dependent physiologic and pathologic stimulus-metabolic response coupling in hepatocytes.Cell calcium · 2012Review
- ATP release after partial hepatectomy regulates liver regeneration in the rat.Journal of hepatology · 2010Article
- The role of purinergic signaling in the liver and in transplantation: effects of extracellular nucleotides on hepatic graft vascular injury, rejection and metabolism.Frontiers in bioscience : a journal and virtual library · 2008Review
- Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8.American journal of physiology. Gastrointestinal and liver physiology · 2007Article
- P2X4, P2Y1 and P2Y2 receptors on rat alveolar macrophages.British journal of pharmacology · 2003Article
- ADP stimulation of inositol phosphates in hepatocytes: role of conversion to ATP and stimulation of P2Y2 receptors.British journal of pharmacology · 2003Article
- Expression of the P2Y1 nucleotide receptor in chick muscle: its functional role in the regulation of acetylcholinesterase and acetylcholine receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- P2 purinoceptor-mediated control of rat cerebral (pial) microvasculature; contribution of P2X and P2Y receptors.The Journal of physiology · 2000Article
- Switching from simple to complex oscillations in calcium signaling.Biophysical journal · 2000Article
- Evidence that 2-methylthioATP and 2-methylthioADP are both agonists at the rat hepatocyte P2Y(1) receptor.British journal of pharmacology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have indicated the expression of multiple P2Y receptors by rat hepatocytes although they have not been identified. Here we show by reverse transcriptase-polymerase chain reaction (RT - PCR) that rat hepatocytes express mRNA encoding all of the four cloned rat P2Y receptors (P2Y(1), P2Y(2), P2Y(4) and P2Y(6)). The effects of UTP have been examined on single aequorin-injected rat hepatocytes. The [Ca(2+)](i) transients induced by UTP were indistinguishable from those induced by ATP in the same cell. The modulatory effects of elevated intracellular cyclic AMP concentration were the same on both UTP- and ATP-induced [Ca(2+)](i) transients. UDP, an agonist at the P2Y(6) receptor, failed to induce transients in hepatocytes, indicating that functional P2Y(6) receptors coupled to increased [Ca(2+)](i) are not expressed. The transients evoked by ADP were more sensitive to inhibition by suramin than those induced by either ATP or UTP. Within an individual cell, the transients induced by ATP and UTP were inhibited by the same concentration of suramin. This sensitivity of ATP and UTP responses to suramin suggests action through P2Y(2) rather than P2Y(4) receptors. Co-application of 30 microM pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) caused a decrease in frequency and amplitude of transients induced by ADP. ATP- and UTP-induced transients also displayed a decrease in amplitude in response to addition of PPADS, but this was accompanied by an increase in frequency of transients. In conclusion the data presented here are consistent with the co-expression of P2Y(1) and P2Y(2) receptors by rat hepatocytes.
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