ArticleProceedings of the National Academy of Sciences of the United States of America2000
Protein folding and unfolding on a complex energy landscape.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 121 citations in OpenAlex.
- Molecular characterization ofFrontiers in microbiology · 2023Article
- Probing Denaturation of Protein A via Surface-Enhanced Infrared Absorption Spectroscopy.Biosensors · 2022Article
- How Similar Are Proteins and Origami?Biomolecules · 2022Article
- Identification of Biomolecules Involved in the Adaptation to the Environment of Cold-Loving Microorganisms and Metabolic Pathways for Their Production.Biomolecules · 2021Review
- Internal constraints and arrested relaxation in main-chain nematic elastomers.Nature communications · 2021Article
- Temperature-Jump 2D IR Spectroscopy with Intensity-Modulated CW Optical Heating.The journal of physical chemistry. B · 2020Article
- Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2018Article
- When fast is better: protein folding fundamentals and mechanisms from ultrafast approaches.The Biochemical journal · 2016Review
- A theoretical study of the unfolding pathway of reduced human serum albumin.Journal of molecular modeling · 2015Article
- Fast protein folding kinetics.Quarterly reviews of biophysics · 2014Review
- Using VIPT-jump to distinguish between different folding mechanisms: application to BBL and a Trpzip.Journal of the American Chemical Society · 2013Article
- The principle of stationary action in biophysics: stability in protein folding.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2013Article
- Force-clamp analysis techniques give highest rank to stretched exponential unfolding kinetics in ubiquitin.Biophysical journal · 2012Article
- Early turn formation and chain collapse drive fast folding of the major cold shock protein CspA of Escherichia coli.Biochemistry · 2012Article
- Is there an en route folding intermediate for Cold shock proteins?Protein science : a publication of the Protein Society · 2012Article
- Di-Cysteine S,S-Tetrazine: A Potential Ultra-fast Photochemical Trigger to Explore the Early Events of Peptide/Protein Folding.Journal of photochemistry and photobiology. A, Chemistry · 2012Article
- Differential ordering of the protein backbone and side chains during protein folding revealed by site-specific recombinant infrared probes.Journal of the American Chemical Society · 2011Article
- Thermal-induced dissociation and unfolding of homodimeric DsbC revealed by temperature-jump time-resolved infrared spectra.Biophysical journal · 2009Article
- Temperature and chemical denaturant dependence of forced unfolding of titin I27.The journal of physical chemistry. B · 2009Article
- A one-dimensional free energy surface does not account for two-probe folding kinetics of protein alpha(3)D.The Journal of chemical physics · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent theories of protein folding suggest that individual proteins within a large ensemble may follow different routes in conformation space from the unfolded state toward the native state and vice versa. Herein, we introduce a new type of kinetics experiment that shows how different unfolding pathways can be selected by varying the initial reaction conditions. The relaxation kinetics of the major cold shock protein of Escherichia coli (CspA) in response to a laser-induced temperature jump are exponential for small temperature jumps, indicative of folding through a two-state mechanism. However, for larger jumps, the kinetics become strongly nonexponential, implying the existence of multiple unfolding pathways. We provide evidence that both unfolding across an energy barrier and diffusive downhill unfolding can occur simultaneously in the same ensemble and provide the experimental requirements for these to be observed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.