ArticleProceedings of the National Academy of Sciences of the United States of America2000
The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 153 citations in OpenAlex.
- Cell-type-specific immune programs orchestrate spatial defense in the Arabidopsis leaf epidermis.Nature communications · 2026Article
- Humidity-driven ABA depletion determines plant-pathogen competition for leaf water.Nature communications · 2025Article
- RetS-mediated environmental sensing coordinates TetR-dependent regulation of type III secretion system and virulence inApplied and environmental microbiology · 2025Article
- Pseudomonas syringae subpopulations cooperate by coordinating flagellar and type III secretion spatiotemporal dynamics to facilitate plant infection.Nature microbiology · 2025Article
- Roles of microbiota in autoimmunity in Arabidopsis leaves.Nature plants · 2024Article
- Comparative sequence analysis of pPATH pathogenicity plasmids inFrontiers in plant science · 2023Article
- The OmpR-like Transcription Factor as a Negative Regulator ofInternational journal of molecular sciences · 2022Article
- Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation.Cell & bioscience · 2022Article
- EssentialInternational journal of molecular sciences · 2022Article
- Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.Cell host & microbe · 2022Article
- Comparative Genomics ofFrontiers in microbiology · 2022Article
- Retrieving theFrontiers in microbiology · 2022Article
- Regulation of theMicroorganisms · 2021Review
- Analysis of HrpG regulons and HrpG-interacting proteins by ChIP-seq and affinity proteomics in Xanthomonas campestris.Molecular plant pathology · 2020Article
- Comparative genomics reveal pathogenicity-related loci in Pseudomonas syringae pv. actinidiae biovar 3.Molecular plant pathology · 2019Article
- A NaMolecular plant pathology · 2019Article
- Two components of the rhpPC operon coordinately regulate the type III secretion system and bacterial fitness in Pseudomonas savastanoi pv. phaseolicola.PLoS pathogens · 2019Article
- Article
- Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp.BMC genomics · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of pili in the transfer process is not understood and is a matter of considerable debate. The Hrp pilus is assembled by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato (Pst) strain DC3000. In this study, we show that the hrpA gene, which encodes the major subunit of the Hrp pilus, is required for secretion of putative virulence proteins, such as HrpW and AvrPto. In addition, the hrpA gene is required for full expression of genes that encode regulatory, secretion, and effector proteins of the type III secretion system. hrpA-mediated gene regulation apparently is through effect on the mRNA level of two previously characterized regulatory genes, hrpR and hrpS. Ectopic expression of the hrpRS gene operon restored gene expression, but not protein secretion, in the hrpA mutant. Three single amino acid mutations at the HrpA carboxyl terminus were identified that affect the secretion or regulatory function of the HrpA protein. These results define an essential role of the Hrp pilus structural gene in protein secretion and coordinate regulation of the type III secretion system in Pst DC3000.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.