ArticleProceedings of the National Academy of Sciences of the United States of America2000
The cellulose synthase gene of Dictyostelium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 95 citations in OpenAlex.
- The Proteome ofProteomes · 2026Article
- Multi-color fluorescence live-cell imaging in Dictyostelium discoideum.Cell structure and function · 2024Article
- On the Mechanism of the Ionizing Radiation-Induced Degradation and Recycling of Cellulose.Polymers · 2023Review
- Chromosome-level genomes of multicellular algal sisters to land plants illuminate signaling network evolution.bioRxiv : the preprint server for biology · 2023Article
- Weaving of bacterial cellulose by the Bcs secretion systems.FEMS microbiology reviews · 2022Review
- Complete Genome Sequence ofGenome announcements · 2017Article
- Early nucleolar disorganization in Dictyostelium cell death.Cell death & disease · 2017Article
- Progress and perspectives in signal transduction, actin dynamics, and movement at the cell and tissue level: lessons fromInterface focus · 2016Review
- Ploidy effect and genetic architecture exploration of stalk traits using DH and its corresponding haploid populations in maize.BMC plant biology · 2016Article
- Genetic control of morphogenesis in Dictyostelium.Developmental biology · 2015Review
- Evidence for land plant cell wall biosynthetic mechanisms in charophyte green algae.Annals of botany · 2014Article
- Article
- The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium.Nature · 2012Article
- A polarized epithelium organized by beta- and alpha-catenin predates cadherin and metazoan origins.Science (New York, N.Y.) · 2011Article
- Update on mechanisms of plant cell wall biosynthesis: how plants make cellulose and other (1->4)-β-D-glycans.Plant physiology · 2011Review
- Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum.Genome biology · 2011Article
- Dictyostelium amoebae and neutrophils can swim.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Autophagic cell death in Dictyostelium requires the receptor histidine kinase DhkM.Molecular biology of the cell · 2010Article
- Characterization of the Roco protein family in Dictyostelium discoideum.Eukaryotic cell · 2010Article
- Evidence for a "wattle and daub" model of the cyst wall of entamoeba.PLoS pathogens · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cellulose is a major component of the extracellular matrices formed during development of the social amoeba, Dictyostelium discoideum. We isolated insertional mutants that failed to accumulate cellulose and had no cellulose synthase activity at any stage of development. Development proceeded normally in the null mutants up to the beginning of stalk formation, at which point the culminating structures collapsed onto themselves, then proceeded to attempt culmination again. No spores or stalk cells were ever made in the mutants, with all cells eventually lysing. The predicted product of the disrupted gene (dcsA) showed significant similarity to the catalytic subunit of cellulose synthases found in bacteria. Enzyme activity and normal development were recovered in strains transformed with a construct expressing the intact dcsA gene. Growing amoebae carrying the construct accumulated the protein product of dcsA, but did not make cellulose until they had developed for at least 10 hr. These studies show directly that the product of dcsA is necessary, but not sufficient, for synthesis of cellulose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.