ArticleMolecular biology of the cell2000
Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.The Journal of cell biology · 2024Article
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.bioRxiv : the preprint server for biology · 2023Article
- Nuclear Localization of G3BP6 Is Essential for the Flowering Transition inBiomolecules · 2023Article
- A Pan-Cancer Analysis of the Oncogenic Role of Nuclear Transport Factor 2 in Human Cancers.Frontiers in oncology · 2022Article
- Methods to separate nuclear soluble fractions reflecting localizations in living cells.iScience · 2021Article
- A role for Mog1 in H2Bub1 and H3K4me3 regulation affecting RNAPII transcription and mRNA export.EMBO reports · 2018Article
- Comparative analysis of plant MKK gene family reveals novel expansion mechanism of the members and sheds new light on functional conservation.BMC genomics · 2018Article
- Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport.The Journal of cell biology · 2013Article
- Nuclear-cytoplasmic trafficking of NTF2, the nuclear import receptor for the RanGTPase, is subjected to regulation.PloS one · 2012Article
- Function of nuclear transport factor 2 and Ran in the 20E signal transduction pathway in the cotton bollworm, Helicoverpa armigera.BMC cell biology · 2010Article
- Nucleocytoplasmic transport: a thermodynamic mechanism.HFSP journal · 2009Article
- Overexpression of nuclear transport factor 2 may protect against diabetic retinopathy.Molecular vision · 2009Article
- Hyperosmotic stress signaling to the nucleus disrupts the Ran gradient and the production of RanGTP.Molecular biology of the cell · 2007Article
- Significant proportions of nuclear transport proteins with reduced intracellular mobilities resolved by fluorescence correlation spectroscopy.Journal of molecular biology · 2007Article
- Crosstalk between the actin cytoskeleton and Ran-mediated nuclear transport.BMC cell biology · 2005Article
- Constitutive and IFN-gamma-induced nuclear import of STAT1 proceed through independent pathways.The EMBO journal · 2002Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
- Calreticulin Is a receptor for nuclear export.The Journal of cell biology · 2001Article
- The interaction between Ran and NTF2 is required for cell cycle progression.Molecular biology of the cell · 2000Article
- RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.Molecular and cellular biology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear transport factor 2 (NTF2) is a soluble transport protein originally identified by its ability to stimulate nuclear localization signal (NLS)-dependent protein import in digitonin-permeabilized cells. NTF2 has been shown to bind nuclear pore complex proteins and the GDP form of Ran in vitro. Recently, it has been reported that NTF2 can stimulate the accumulation of Ran in digitonin-permeabilized cells. Evidence that NTF2 directly mediates Ran import or that NTF2 is required to maintain the nuclear concentration of Ran in living cells has not been obtained. Here we show that cytoplasmic injection of anti-NTF2 mAbs resulted in a dramatic relocalization of Ran to the cytoplasm. This provides the first evidence that NTF2 regulates the distribution of Ran in vivo. Moreover, anti-NTF2 mAbs inhibited nuclear import of both Ran and NLS-containing protein in vitro, suggesting that NTF2 stimulates NLS-dependent protein import by driving the nuclear accumulation of Ran. We also show that biotinylated NTF2-streptavidin microinjected into the cytoplasm accumulated at the nuclear envelope, indicating that NTF2 can target a binding partner to the nuclear pore complex. Taken together, our data show that NTF2 is an essential regulator of the Ran distribution in living cells and that NTF2-mediated Ran nuclear import is required for NLS-dependent protein import.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.