ArticleMolecular biology of the cell2000
The secretory pathway mediates localization of the cell polarity regulator Aip3p/Bud6p.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Bil2 Is a Novel Inhibitor of the Yeast Formin Bnr1 Required for Proper Actin Cable Organization and Polarized Secretion.Frontiers in cell and developmental biology · 2021Article
- YFR016c/Aip5 is part of an actin nucleation complex in yeast.Biology open · 2019Article
- Integrated control of formin-mediated actin assembly by a stationary inhibitor and a mobile activator.The Journal of cell biology · 2018Article
- Article
- Cell-cycle regulation of formin-mediated actin cable assembly.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.The Journal of cell biology · 2013Article
- Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites.Eukaryotic cell · 2012Article
- Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.Molecular biology of the cell · 2011Article
- Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.Molecular biology of the cell · 2010Article
- Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties.BMC genomics · 2010Article
- A biochemical genomics screen for substrates of Ste20p kinase enables the in silico prediction of novel substrates.PloS one · 2009Article
- Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.Eukaryotic cell · 2007Article
- Yeast formins Bni1 and Bnr1 utilize different modes of cortical interaction during the assembly of actin cables.Molecular biology of the cell · 2007Article
- Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6.Eukaryotic cell · 2006Article
- Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis.The Journal of cell biology · 2005Article
- Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth.The Journal of cell biology · 2005Article
- Cyclical regulation of the exocyst and cell polarity determinants for polarized cell growth.Molecular biology of the cell · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aip3p/Bud6p is a regulator of cell and cytoskeletal polarity in Saccharomyces cerevisiae that was previously identified as an actin-interacting protein. Actin-interacting protein 3 (Aip3p) localizes at the cell cortex where cytoskeleton assembly must be achieved to execute polarized cell growth, and deletion of AIP3 causes gross defects in cell and cytoskeletal polarity. We have discovered that Aip3p localization is mediated by the secretory pathway. Mutations in early- or late-acting components of the secretory apparatus lead to Aip3p mislocalization. Biochemical data show that a pool of Aip3p is associated with post-Golgi secretory vesicles. An investigation of the sequences within Aip3p necessary for Aip3p localization has identified a sequence within the N terminus of Aip3p that is sufficient for directing Aip3p localization. Replacement of the N terminus of Aip3p with a homologous region from a Schizosaccharomyces pombe protein allows for normal Aip3p localization, indicating that the secretory pathway-mediated Aip3p localization pathway is conserved. Delivery of Aip3p also requires the type V myosin motor Myo2p and its regulatory light-chain calmodulin. These data suggest that one function of calmodulin is to activate Myo2p's activity in the secretory pathway; this function is likely the polarized movement of late secretory vesicles and associated Aip3p on actin cables.
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