ArticleMolecular biology of the cell2000
Detergent-insoluble GPI-anchored proteins are apically sorted in fischer rat thyroid cells, but interference with cholesterol or sphingolipids differentially affects detergent insolubility and apical sorting.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 116 citations in OpenAlex.
- Calcium-binding Cab45 regulates the polarized apical secretion of soluble proteins in epithelial cells.Molecular biology of the cell · 2023Article
- Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Membrane Heterogeneity Beyond the Plasma Membrane.Frontiers in cell and developmental biology · 2020Review
- With or without rafts? Alternative views on cell membranes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Ligand binding alters dimerization and sequestering of urokinase receptors in raft-mimicking lipid mixtures.Biophysical journal · 2014Article
- Trafficking to the apical and basolateral membranes in polarized epithelial cells.Journal of the American Society of Nephrology : JASN · 2014Review
- Membrane raft association is a determinant of plasma membrane localization.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Biology of tissue factor pathway inhibitor.Blood · 2014Review
- Anti-tetherin activities of HIV-1 Vpu and Ebola virus glycoprotein do not involve removal of tetherin from lipid rafts.Journal of virology · 2012Article
- N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.Molecular biology of the cell · 2011Article
- Early response to ErbB2 over-expression in polarized Caco-2 cells involves partial segregation from ErbB3 by relocalization to the apical surface and initiation of survival signaling.Journal of cellular biochemistry · 2010Article
- Apical localization of PMCA2w/b is lipid raft-dependent.Biochemical and biophysical research communications · 2009Article
- Selective roles for cholesterol and actin in compartmentalization of different proteins in the Golgi and plasma membrane of polarized cells.The Journal of biological chemistry · 2008Article
- Functional coupling of Gs and CFTR is independent of their association with lipid rafts in epithelial cells.Pflugers Archiv : European journal of physiology · 2008Article
- Dimeric PKD regulates membrane fission to form transport carriers at the TGN.The Journal of cell biology · 2007Article
- Polarization-dependent selective transport to the apical membrane by KIF5B in MDCK cells.Developmental cell · 2007Article
- Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.The Journal of cell biology · 2006Article
- GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells.The Journal of cell biology · 2006Article
- Rotavirus assembly: an alternative model that utilizes an atypical trafficking pathway.Current topics in microbiology and immunology · 2006Review
- Relationships between EGFR signaling-competent and endocytosis-competent membrane microdomains.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to Madin-Darby canine kidney cells, Fischer rat thyroid cells deliver the majority of endogenous glycosylphosphatidyl inositol (GPI)-anchored proteins to the basolateral surface. However, we report here that the GPI proteins Placental Alkaline Phosphatase (PLAP) and Neurotrophin Receptor-Placental Alkaline Phosphatase (NTR-PLAP) are apically localized in transfected Fischer rat thyroid cells. In agreement with the "raft hypothesis," which postulates the incorporation of GPI proteins into glycosphingolipids and cholesterol-enriched rafts, we found that both of these proteins were insoluble in Triton X-100 and floated into the lighter fractions of sucrose density gradients. However, disruption of lipid rafts by removal of cholesterol did not cause surface missorting of PLAP and NTR-PLAP, and the altered surface sorting of these proteins after Fumonisin B1 treatment did not correlate with reduced levels in Triton X-100 -insoluble fractions. Furthermore, in contrast to the GPI-anchored forms of both of these proteins, the secretory and transmembrane forms (in the absence of a basolateral cytoplasmic signal) were sorted to the apical surface without association with lipid microdomains. Together, these data demonstrate that the GPI anchor is required to mediate raft association but is not sufficient to determine apical sorting. They also suggest that signals present in the ectodomain of the proteins play a major role and that lipid rafts may facilitate the recognition of these signals in the trans-Golgi network, even though they are not required for apical sorting.
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