ArticleMolecular biology of the cell2000
Sbe2p and sbe22p, two homologous Golgi proteins involved in yeast cell wall formation.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Avl9 defines a family of DENN domain GTPase-activating proteins.The Journal of cell biology · 2026Article
- The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2026Article
- Avl9 defines a family of GTPase-activating proteins that regulate diverse cell biological functions.bioRxiv : the preprint server for biology · 2026Article
- Emerging Roles of Exocyst Complex in Fungi: A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- Exploring whole-genome duplicate gene retention with complex genetic interaction analysis.Science (New York, N.Y.) · 2020Article
- Characterization of the Probiotic Yeast Saccharomyces boulardii in the Healthy Mucosal Immune System.PloS one · 2016Article
- Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway.Molecular and cellular biology · 2015Article
- Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins.Molecular cell · 2014Article
- SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces cerevisiae and produced dependently on oxygen level.PloS one · 2013Article
- Genome-wide association analysis of clinical vs. nonclinical origin provides insights into Saccharomyces cerevisiae pathogenesis.Molecular ecology · 2011Article
- A genome-wide immunodetection screen in S. cerevisiae uncovers novel genes involved in lysosomal vacuole function and morphology.PloS one · 2011Article
- The exomer coat complex transports Fus1p to the plasma membrane via a novel plasma membrane sorting signal in yeast.Molecular biology of the cell · 2009Article
- ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae.The Journal of membrane biology · 2009Article
- Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast.The Journal of cell biology · 2006Article
- 2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeastWorld journal of microbiology & biotechnology · 2006Article
- Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- Roles of phosphoinositides and of Spo14p (phospholipase D)-generated phosphatidic acid during yeast sporulation.Molecular biology of the cell · 2004Article
- Specific protein targeting during cell differentiation: polarized localization of Fus1p during mating depends on Chs5p in Saccharomyces cerevisiae.Eukaryotic cell · 2003Article
- A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p.Molecular biology of the cell · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The cell wall of fungal cells is important for cell integrity and cell morphogenesis and protects against harmful environmental conditions. The yeast cell wall is a complex structure consisting mainly of mannoproteins, glucan, and chitin. The molecular mechanisms by which the cell wall components are synthesized and transported to the cell surface are poorly understood. We have identified and characterized two homologous yeast proteins, Sbe2p and Sbe22p, through their suppression of a chs5 spa2 mutant strain defective in chitin synthesis and cell morphogenesis. Although sbe2 and sbe22 null mutants are viable, sbe2 sbe22 cells display several phenotypes indicative of defects in cell integrity and cell wall structure. First, sbe2 sbe22 cells display a sorbitol-remediable lysis defect at 37 degrees C and are hypersensitive to SDS and calcofluor. Second, electron microscopic analysis reveals that sbe2 sbe22 cells have an aberrant cell wall structure with a reduced mannoprotein layer. Finally, immunofluorescence experiments reveal that in small-budded cells, sbe2 sbe22 mutants mislocalize Chs3p, a protein involved in chitin synthesis. In addition, sbe2 sbe22 diploids have a bud-site selection defect, displaying a random budding pattern. A Sbe2p-GFP fusion protein localizes to cytoplasmic patches, and Sbe2p cofractionates with Golgi proteins. Deletion of CHS5, which encodes a Golgi protein involved in the transport of Chs3p to the cell periphery, is lethal in combination with disruption of SBE2 and SBE22. Thus, we suggest a model in which Sbe2p and Sbe22p are involved in the transport of cell wall components from the Golgi apparatus to the cell surface periphery in a pathway independent of Chs5p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.