ArticleMolecular biology of the cell2000
Reconstitution of ATP-dependent movement of endocytic vesicles along microtubules in vitro: an oscillatory bidirectional process.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 95 citations in OpenAlex.
- From oversight to insight: the curious case of the endothelial insulin receptor.Blood vessels, thrombosis & hemostasis · 2026Review
- Paxillin regulates Rab5-mediated vesicle motility through modulating microtubule acetylation.Molecular biology of the cell · 2023Article
- IL-36γ is secreted through an unconventional pathway using the Gasdermin D and P2X7R membrane pores.Frontiers in immunology · 2022Article
- Microtubules in Influenza Virus Entry and Egress.Viruses · 2020Review
- Rat Organic Anion Transport Protein 1A1 Interacts Directly With Organic Anion Transport Protein 1A4 Facilitating Its Maturation and Trafficking to the Hepatocyte Plasma Membrane.Hepatology (Baltimore, Md.) · 2019Article
- Article
- The Na(+)-Taurocholate Cotransporting Polypeptide Traffics with the Epidermal Growth Factor Receptor.Traffic (Copenhagen, Denmark) · 2016Article
- Blocking ESCRT-mediated envelopment inhibits microtubule-dependent trafficking of alphaherpesviruses in vitro.Journal of virology · 2014Article
- Organic anion uptake by hepatocytes.Comprehensive Physiology · 2014Review
- Rab1a regulates sorting of early endocytic vesicles.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Oatp1a1 requires PDZK1 to traffic to the plasma membrane by selective recruitment of microtubule-based motor proteins.Drug metabolism and disposition: the biological fate of chemicals · 2014Article
- Lipid droplets purified from Drosophila embryos as an endogenous handle for precise motor transport measurements.Biophysical journal · 2013Article
- Article
- Measuring molecular motor forces in vivo: implications for tug-of-war models of bidirectional transport.Biophysical journal · 2012Article
- Microtubule- and dynein-dependent nuclear trafficking of rhesus rhadinovirus in rhesus fibroblasts.Journal of virology · 2012Article
- The enlarged lysosomes in beige j cells result from decreased lysosome fission and not increased lysosome fusion.Traffic (Copenhagen, Denmark) · 2012Article
- Bidirectional intracellular transport: utility and mechanism.Biochemical Society transactions · 2011Article
- Endosome maturation.The EMBO journal · 2011Review
- In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.The Journal of biological chemistry · 2011Article
- Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles.Journal of cell science · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have previously used the asialoglycoprotein receptor system to elucidate the pathway of hepatocytic processing of ligands such as asialoorosomucoid (ASOR). These studies suggested that endocytic vesicles bind to and travel along microtubules under the control of molecular motors such as cytoplasmic dynein. We now report reconstitution of this process in vitro with the use of a microscope assay to observe the interaction of early endocytic vesicles containing fluorescent ASOR with fluorescent microtubules. We find that ASOR-containing endosomes bind to microtubules and translocate along them in the presence of ATP. This represents the first time that mammalian endosomes containing a well-characterized ligand have been directly observed to translocate on microtubules in vitro. The endosome movement does not require cytosol or exogenous motor protein, is oscillatory, and is directed toward the plus and minus ends at equal frequencies. We also observe endosomes being stretched in opposite directions along microtubules, suggesting that microtubules could provide a mechanical basis for endocytic sorting events. The movement of endosomes in vitro is consistent with the hypothesis that microtubules actively participate in the sorting and distribution of endocytic contents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.