ArticleProceedings of the National Academy of Sciences of the United States of America2000
Efficient studies of long-distance Bmp5 gene regulation using bacterial artificial chromosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 65 citations in OpenAlex.
- Complex Regulatory Interactions at GDF5 Shape Joint Morphology and Osteoarthritis Disease Risk.Arthritis & rheumatology (Hoboken, N.J.) · 2025Article
- Ear pinna growth and differentiation is conserved in murids and requires BMP signaling for chondrocyte proliferation.Development (Cambridge, England) · 2025Article
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- Article
- An integrative genomic analysis of the Longshanks selection experiment for longer limbs in mice.eLife · 2019Article
- A novel enhancer near theeLife · 2018Article
- Heads, Shoulders, Elbows, Knees, and Toes: Modular Gdf5 Enhancers Control Different Joints in the Vertebrate Skeleton.PLoS genetics · 2016Article
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- Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elements.Developmental biology · 2013Article
- Association of a BMP5 microsatellite with knee osteoarthritis: case-control study.Arthritis research & therapy · 2012Article
- Mutation of Rubie, a novel long non-coding RNA located upstream of Bmp4, causes vestibular malformation in mice.PloS one · 2012Article
- Association of a functional microsatellite within intron 1 of the BMP5 gene with susceptibility to osteoarthritis.BMC medical genetics · 2009Article
- Identification of an ancient Bmp4 mesoderm enhancer located 46 kb from the promoter.Developmental biology · 2009Article
- Shaping skeletal growth by modular regulatory elements in the Bmp5 gene.PLoS genetics · 2008Article
- Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Dominant negative Bmp5 mutation reveals key role of BMPs in skeletal response to mechanical stimulation.BMC developmental biology · 2008Article
- Fate mapping using Cited1-CreERT2 mice demonstrates that the cap mesenchyme contains self-renewing progenitor cells and gives rise exclusively to nephronic epithelia.Developmental biology · 2008Article
- Differential gene expression in the peripheral zone compared to the transition zone of the human prostate gland.Prostate cancer and prostatic diseases · 2008Article
- BMP-5 expression increases during chondrocyte differentiation in vivo and in vitro and promotes proliferation and cartilage matrix synthesis in primary chondrocyte cultures.Journal of cellular physiology · 2008Article
- A mouse transgene drives embryonic dorsal posterior commissure expression.Transgenic research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulatory regions surrounding many genes may be large and difficult to study using standard transgenic approaches. Here we describe the use of bacterial artificial chromosome clones to rapidly survey hundreds of kilobases of DNA for potential regulatory sequences surrounding the mouse bone morphogenetic protein-5 (Bmp5) gene. Simple coinjection of large insert clones with lacZ reporter constructs recapitulates all of the sites of expression observed previously with numerous small constructs covering a large, complex regulatory region. The coinjection approach has made it possible to rapidly survey other regions of the Bmp5 gene for potential control elements, to confirm the location of several elements predicted from previous expression studies using regulatory mutations at the Bmp5 locus, to test whether Bmp5 control regions act similarly on endogenous and foreign promoters, and to show that Bmp5 control elements are capable of rescuing phenotypic effects of a Bmp5 deficiency. This rapid approach has identified new Bmp5 control regions responsible for controlling the development of specific anatomical structures in the vertebrate skeleton. A similar approach may be useful for studying complex control regions surrounding many other genes important in embryonic development and human disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.