ArticleProceedings of the National Academy of Sciences of the United States of America2000
The golgi-associated COPI-coated buds and vesicles contain beta/gamma -actin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- ER/Golgi trafficking is facilitated by unbranched actin filaments containing Tpm4.2.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Actin Filaments Are Involved in the Coupling of V0-V1 Domains of Vacuolar H+-ATPase at the Golgi Complex.The Journal of biological chemistry · 2016Article
- The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons.Molecular biology of the cell · 2014Article
- Regulation of calcium channels in smooth muscle: new insights into the role of myosin light chain kinase.Channels (Austin, Tex.) · 2014Review
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.Molecular biology of the cell · 2012Article
- Regulation of late endosomal/lysosomal maturation and trafficking by cortactin affects Golgi morphology.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Transport of influenza virus neuraminidase (NA) to host cell surface is regulated by ARHGAP21 and Cdc42 proteins.The Journal of biological chemistry · 2012Article
- Involvement of the Rho-mDia1 pathway in the regulation of Golgi complex architecture and dynamics.Molecular biology of the cell · 2011Article
- Article
- RNAi screen of Salmonella invasion shows role of COPI in membrane targeting of cholesterol and Cdc42.Molecular systems biology · 2011Article
- ARF1-mediated actin polymerization produces movement of artificial vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Mechanism of Akt1 inhibition of breast cancer cell invasion reveals a protumorigenic role for TSC2.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins.Microbiology and molecular biology reviews : MMBR · 2004Review
- LIMK1 regulates Golgi dynamics, traffic of Golgi-derived vesicles, and process extension in primary cultured neurons.Molecular biology of the cell · 2004Article
- Targeting of a tropomyosin isoform to short microfilaments associated with the Golgi complex.Molecular biology of the cell · 2004Article
- Loss of myosin VI reduces secretion and the size of the Golgi in fibroblasts from Snell's waltzer mice.The EMBO journal · 2003Article
- Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport.Molecular biology of the cell · 2003Article
- Distinct effect of actin cytoskeleton disassembly on exo- and endocytic events in a membrane patch of rat melanotrophs.The Journal of physiology · 2002Article
- Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been shown previously that the morphology and subcellular positioning of the Golgi complex is controlled by actin microfilaments. To further characterize the association between actin microfilaments and the Golgi complex, we have used the Clostridium botulinum toxins C2 and C3, which specifically inhibit actin polymerization and cause depolymerization of F-actin in intact cells by the ADP ribosylation of G-actin monomers and the Rho small GTP-binding protein, respectively. Normal rat kidney cells treated with C2 showed that disruption of the actin and the collapse of the Golgi complex occurred concomitantly. However, when cells were treated with C3, the actin disassembly was observed without any change in the organization of the Golgi complex. The absence of the involvement of Rho was further confirmed by the treatment with lysophosphatidic acid or microinjection with the constitutively activated form of RhoA, both of which induced the stress fiber formation without affecting the Golgi complex. Immunogold electron microscopy in normal rat kidney cells revealed that beta- and gamma-actin isoforms were found in Golgi-associated COPI-coated buds and vesicles. Taken together, the results suggest that the Rho signaling pathway does not directly regulate Golgi-associated actin microfilaments, and that beta- and gamma-actins might be involved in the formation and/or transport of Golgi-derived vesicular or tubular intermediates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.