ArticlePlant physiology2000
Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 151 citations in OpenAlex.
- An antifungal effector from a plant-parasitic nematode modulates host fungal community composition and supports ecological fitness.NPJ biofilms and microbiomes · 2026Article
- A transcription factor ensemble orchestrates bundle sheath expression in rice.Nature communications · 2025Article
- Decoupling the pleiotropic effects of VRT-A2 during reproductive development enhances wheat grain length and weight.The Plant cell · 2025Article
- Sequence-dependent and -independent effects of intron-mediated enhancement learned from thousands of random introns.Nucleic acids research · 2025Article
- High-quality genome of a modern soybean cultivar and resequencing of 547 accessions provide insights into the role of structural variation.Nature genetics · 2024Article
- Splicing-mediated activation of SHAGGY-like kinases underpinning carbon partitioning in Arabidopsis seeds.The Plant cell · 2022Article
- Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity.Frontiers in plant science · 2022Article
- Expression Elements Derived From Plant Sequences Provide Effective Gene Expression Regulation and New Opportunities for Plant Biotechnology Traits.Frontiers in plant science · 2021Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- First Come, First Served: Sui Generis Features of the First Intron.Plants (Basel, Switzerland) · 2020Review
- Sex-specific impact of inbreeding on pathogen load in the striped dolphin.Proceedings. Biological sciences · 2020Article
- An intron-derived motif strongly increases gene expression from transcribed sequences through a splicing independent mechanism in Arabidopsis thaliana.Scientific reports · 2019Article
- A universal approach to investigate circRNA protein coding function.Scientific reports · 2019Article
- Intron-mediated regulation of β-tubulin genes expression affects the sensitivity to carbendazim in Fusarium graminearum.Current genetics · 2019Article
- Liriodendron genome sheds light on angiosperm phylogeny and species-pair differentiation.Nature plants · 2019Article
- Tracing the evolution of the heterotrimeric G protein α subunit in Metazoa.BMC evolutionary biology · 2018Article
- Requirement for intron structures in activating theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Trichoderma virens β-glucosidase I (BGLI) gene; expression in Saccharomyces cerevisiae including docking and molecular dynamics studies.BMC microbiology · 2017Article
- Intron DNA Sequences Can Be More Important Than the Proximal Promoter in Determining the Site of Transcript Initiation.The Plant cell · 2017Article
- Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Either of the first two introns of the Arabidopsis tryptophan pathway gene PAT1 elevates mRNA accumulation from a PAT1:beta-glucuronidase (GUS) fusion roughly 5-fold without affecting the rate of PAT1:GUS transcription. To further explore the mechanism of this intron-mediated enhancement of gene expression, we wanted to determine whether splicing or specific intron sequences were necessary. In-frame derivatives of PAT1 intron 1, whose splicing was prevented by a point mutation or large deletions, were able to increase mRNA accumulation from a PAT1:GUS fusion, demonstrating that splicing per se is not required. Furthermore, each of a series of introns containing overlapping deletions that together span PAT1 intron 1 increased PAT1:GUS mRNA accumulation as much as the full-length intron did, indicating that all intron sequences are individually dispensable for this phenomenon. These results eliminate the simple idea that this intron stimulates mRNA accumulation via a unique RNA-stabilizing sequence or through the completed act of splicing. However, they are consistent with a possible role for redundant intron sequence elements or an association of the pre-mRNA with the spliceosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.