ArticleThe Biochemical journal2000
Interaction between the homeodomain proteins Cdx2 and HNF1alpha mediates expression of the lactase-phlorizin hydrolase gene.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 113 citations in OpenAlex.
- Defective HNF1A hinders GLI3 processing favoring duodenal versus pancreatic fate, thus leading to intestinal elongation in vivo.Genes & development · 2026Article
- O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation.Molecular & cellular proteomics : MCP · 2023Article
- Genetics of Lactose Intolerance: An Updated Review and Online Interactive World Maps of Phenotype and Genotype Frequencies.Nutrients · 2020Review
- Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression.Nucleic acids research · 2017Article
- Hepatocyte nuclear factor 1 coordinates multiple processes in a model of intestinal epithelial cell function.Biochimica et biophysica acta · 2016Article
- CDX2 homeoprotein is involved in the regulation of ST6GalNAc-I gene in intestinal metaplasia.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Transcription factors GATA4 and HNF4A control distinct aspects of intestinal homeostasis in conjunction with transcription factor CDX2.The Journal of biological chemistry · 2015Article
- Differential expression of Homeobox C11 protein in water buffalo Bubalus bubalis and its putative 3D structure.BMC genomics · 2014Article
- Kruppel-like factor 5 controls villus formation and initiation of cytodifferentiation in the embryonic intestinal epithelium.Developmental biology · 2013Article
- Transcriptional networks driving enhancer function in the CFTR gene.The Biochemical journal · 2012Article
- Polycomb repressive complex 2 impedes intestinal cell terminal differentiation.Journal of cell science · 2012Article
- Caudal homeobox protein Cdx-2 cooperates with Wnt pathway to regulate claudin-1 expression in colon cancer cells.PloS one · 2012Article
- The -14010*C variant associated with lactase persistence is located between an Oct-1 and HNF1α binding site and increases lactase promoter activity.Human genetics · 2011Article
- Cdx2 levels modulate intestinal epithelium maturity and Paneth cell development.Gastroenterology · 2011Article
- Genome-wide analysis of CDX2 binding in intestinal epithelial cells (Caco-2).The Journal of biological chemistry · 2010Article
- Cooperation between HNF-1alpha, Cdx2, and GATA-4 in initiating an enterocytic differentiation program in a normal human intestinal epithelial progenitor cell line.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Mapping of HNF4alpha target genes in intestinal epithelial cells.BMC gastroenterology · 2009Article
- Lactose digestion and the evolutionary genetics of lactase persistence.Human genetics · 2009Article
- Proteomic screen defines the hepatocyte nuclear factor 1alpha-binding partners and identifies HMGB1 as a new cofactor of HNF1alpha.Nucleic acids research · 2008Article
- Human acyl-CoA:cholesterol acyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma.The Biochemical journal · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactase-phlorizin hydrolase is a brush-border enzyme which is specifically expressed in the small intestine where it hydrolyses lactose, the main carbohydrate found in milk. We have previously demonstrated in transgenic mice that the tissue-specific and developmental expression of lactase is controlled by a 1 kb upstream region of the pig lactase gene. Two homeodomain transcription factors, caudal-related homeodomain protein (Cdx2) and hepatic nuclear factor 1alpha (HNF1alpha), are known to bind to regulatory cis elements in the promoters for several intestine-specific genes, including lactase, and are present in mammalian intestinal epithelia from an early stage in development. In the present study, we examined whether Cdx2 and HNF1alpha physically interact and co-operatively activate transcription from the lactase-phlorizin hydrolase promoter. We show that the presence of both factors leads to a much higher level of transcription than the sum of the activation by either factor alone. The N-terminal activation domain of Cdx2 is required for maximal synergy with HNF1alpha. With the use of pull-down assays, we demonstrate a direct protein-protein interaction between Cdx2 and HNF1alpha. The interaction domain includes the homeodomain region of both proteins. This is the first demonstration of a functional interaction between two transcription factors involved in the activation of a number of intestine-specific genes. Synergistic interaction between tissue-restricted factors is likely to be an important mechanism for reinforcing developmental and tissue-specific gene expression within the intestine.
Indexed as
Identifiers
10677375PMC1220882W2054810829What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.