ArticleThe Biochemical journal2000
Identification and expression of an allergen Asp f 13 from Aspergillus fumigatus and epitope mapping using human IgE antibodies and rabbit polyclonal antibodies.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Airborne indoor allergen serine proteases and their contribution to sensitisation and activation of innate immunity in allergic airway disease.European respiratory review : an official journal of the European Respiratory Society · 2024Review
- The Human Cathelicidin Antimicrobial Peptide LL-37 Promotes the Growth of the Pulmonary Pathogen Aspergillus fumigatus.Infection and immunity · 2018Article
- Proteomics as a Tool to Identify New Targets Against Aspergillus and Scedosporium in the Context of Cystic Fibrosis.Mycopathologia · 2018Review
- Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES.The Journal of biological chemistry · 2016Article
- Interference of Aspergillus fumigatus with the immune response.Seminars in immunopathology · 2015Review
- Identification of fibrinogen-binding proteins of Aspergillus fumigatus using proteomic approach.Mycopathologia · 2012Article
- Identification of critical amino acids in an immunodominant IgE epitope of Pen c 13, a major allergen from Penicillium citrinum.PloS one · 2012Article
- Fungal proteases and their pathophysiological effects.Mycopathologia · 2011Review
- Identification, expression and characterisation of a major salivary allergen (Cul s 1) of the biting midge Culicoides sonorensis relevant for summer eczema in horses.International journal for parasitology · 2009Article
- Proteomic and transcriptomic analysis of Aspergillus fumigatus on exposure to amphotericin B.Antimicrobial agents and chemotherapy · 2008Article
- Comprehensive 3D-modeling of allergenic proteins and amino acid composition of potential conformational IgE epitopes.Molecular immunology · 2008Article
- Animal models: an important tool in mycology.Medical mycology · 2007Review
- Inflammatory effect of environmental proteases on airway mucosa.Current allergy and asthma reports · 2007Review
- Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity.Indian journal of clinical biochemistry : IJCB · 2006Article
- Molecular and immunological characterization and IgE epitope mapping of Pen n 18, a major allergen of Penicillium notatum.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Aspergillus genus of fungi is known to be one of the most prevalent aeroallergens. On two-dimensional immunoblotting using patients' sera containing IgE specific for Asp f 13, an allergen with a molecular mass of 33 kDa and a pI of 6.2 was identified. This allergen was also present in A. fumigatus culture filtrates. Furthermore, the sequence of the Asp f 13 cDNA was identical to that for alkaline protease isolated from A. fumigatus and showed 42-49% identity of amino acids with two proteases from P. cyclopium and T. album and with the Pen c 1 allergen from P. citrinum. Asp f 13 coding sequences were expressed in Escherichia coli as a [His](6)-tagged fusion protein which was purified by Ni(2+)-chelate affinity chromatography. Recombinant Asp f 13 was recognized by rabbit polyclonal antibodies against Asp f 13 and by IgE antibodies from subject allergic to A. fumigatus. To identify and characterize the linear epitopes of this allergen, a combination of chemical and enzymatic cleavage and immunoblotting techniques, with subsequent N-terminal sequencing and mass spectrometry, were performed. At least 13 different linear epitopes reacting with the rabbit anti-Asp f 13 antiserum were identified, located throughout the entire molecule. In contrast, IgE from A. fumigatus-sensitive patients bound to three immunodominant epitopes at the C-terminal of the protein.
Indexed as
Identifiers
10677362PMC1220869W1994965979What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.