ArticleThe Journal of clinical investigation2000
Syntaxin 1A is expressed in airway epithelial cells, where it modulates CFTR Cl(-) currents.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 70 citations in OpenAlex.
- Effects of Anti-Fibrotic Drugs on Transcriptome of Peripheral Blood Mononuclear Cells in Idiopathic Pulmonary Fibrosis.International journal of molecular sciences · 2024Article
- Revisiting CFTR Interactions: Old Partners and New Players.International journal of molecular sciences · 2021Review
- Monoubiquitination of syntaxin 3 leads to retrieval from the basolateral plasma membrane and facilitates cargo recruitment to exosomes.Molecular biology of the cell · 2017Article
- AP2 α modulates cystic fibrosis transmembrane conductance regulator function in the human intestine.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2017Article
- Article
- Mechanisms of Intestinal Serotonin Transporter (SERT) Upregulation by TGF-β1 Induced Non-Smad Pathways.PloS one · 2015Article
- Clinical and molecular characterization of the potential CF disease modifier syntaxin 1A.European journal of human genetics : EJHG · 2013Article
- Characterization of CFTR High Expresser cells in the intestine.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- In vitro analysis of PDZ-dependent CFTR macromolecular signaling complexes.Journal of visualized experiments : JoVE · 2012Article
- Physiology of epithelial chloride and fluid secretion.Cold Spring Harbor perspectives in medicine · 2012Article
- Analysis of CFTR interactome in the macromolecular complexes.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Syntaxin 3 is necessary for cAMP- and cGMP-regulated exocytosis of CFTR: implications for enterotoxigenic diarrhea.American journal of physiology. Cell physiology · 2010Article
- DeltaF508 CFTR processing correction and activity in polarized airway and non-airway cell monolayers.Pulmonary pharmacology & therapeutics · 2010Article
- Alpha-AP-2 directs myosin VI-dependent endocytosis of cystic fibrosis transmembrane conductance regulator chloride channels in the intestine.The Journal of biological chemistry · 2010Article
- Regulation of the epithelial sodium channel by membrane trafficking.American journal of physiology. Renal physiology · 2009Review
- The formation of the cAMP/protein kinase A-dependent annexin 2-S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel function.Molecular biology of the cell · 2007Article
- Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR.The Journal of clinical investigation · 2007Article
- Distinct domain-dependent effect of syntaxin1A on amiloride-sensitive sodium channel (ENaC) currents in HT-29 colonic epithelial cells.International journal of biological sciences · 2006Article
- Failure of cAMP agonists to activate rescued deltaF508 CFTR in CFBE41o- airway epithelial monolayers.The Journal of physiology · 2005Article
- Botulinum toxins inhibit the antidiuretic hormone (ADH)-stimulated increase in rabbit cortical collecting-tubule water Permeability.The Journal of membrane biology · 2005Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
The CFTR Cl(-) channel controls salt and water transport across epithelial tissues. Previously, we showed that CFTR-mediated Cl(-) currents in the Xenopus oocyte expression system are inhibited by syntaxin 1A, a component of the membrane trafficking machinery. This negative modulation of CFTR function can be reversed by soluble syntaxin 1A peptides and by the syntaxin 1A binding protein, Munc-18. In the present study, we determined whether syntaxin 1A is expressed in native epithelial tissues that normally express CFTR and whether it modulates CFTR currents in these tissues. Using immunoblotting and immunofluorescence, we observed syntaxin 1A in native gut and airway epithelial tissues and showed that epithelial cells from these tissues express syntaxin 1A at >10-fold molar excess over CFTR. Syntaxin 1A is seen near the apical cell surfaces of human bronchial airway epithelium. Reagents that disrupt the CFTR-syntaxin 1A interaction, including soluble syntaxin 1A cytosolic domain and recombinant Munc-18, augmented cAMP-dependent CFTR Cl(-) currents by more than 2- to 4-fold in mouse tracheal epithelial cells and cells derived from human nasal polyps, but these reagents did not affect CaMK II-activated Cl(-) currents in these cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.