ArticleThe EMBO journal2000
N-acyl homoserine lactone binding to the CarR receptor determines quorum-sensing specificity in Erwinia.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 175 citations in OpenAlex.
- Insights into the Mechanisms of Small Molecule-Induced Agonism and Antagonism of a Quorum Sensing Receptor inJournal of the American Chemical Society · 2025Article
- Autoinducer-fluorophore conjugates enable FRET in LuxR proteins in vitro and in cells.Nature chemical biology · 2022Article
- Article
- Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.FEMS microbiology letters · 2018Article
- Pulse Generation in the Quorum Machinery of Pseudomonas aeruginosa.Bulletin of mathematical biology · 2017Article
- How fast is a collective bacterial state established?PloS one · 2017Article
- Article
- Using surface enhanced Raman scattering to analyze the interactions of protein receptors with bacterial quorum sensing modulators.ACS nano · 2015Article
- Microbial biofilm as a smart material.Sensors (Basel, Switzerland) · 2015Article
- Article
- A bottom-up characterization of transfer functions for synthetic biology designs: lessons from enzymology.Nucleic acids research · 2014Article
- LuxR solos in Photorhabdus species.Frontiers in cellular and infection microbiology · 2014Article
- Structural insights into a novel interkingdom signaling circuit by cartography of the ligand-binding sites of the homologous quorum sensing LuxR-family.International journal of molecular sciences · 2013Article
- N-acyl homoserine lactone-producing Pseudomonas putida strain T2-2 from human tongue surface.Sensors (Basel, Switzerland) · 2013Article
- Kinetic model for signal binding to the Quorum sensing regulator LasR.International journal of molecular sciences · 2013Article
- A design principle of group-level decision making in cell populations.PLoS computational biology · 2013Article
- The Pseudomonas aeruginosa global regulator VqsR directly inhibits QscR to control quorum-sensing and virulence gene expression.Journal of bacteriology · 2012Article
- The GtaR protein negatively regulates transcription of the gtaRI operon and modulates gene transfer agent (RcGTA) expression in Rhodobacter capsulatus.Molecular microbiology · 2012Article
- Classifying the topology of AHL-driven quorum sensing circuits in proteobacterial genomes.Sensors (Basel, Switzerland) · 2012Review
- Quorum sensing and expression of virulence in pectobacteria.Sensors (Basel, Switzerland) · 2012Review
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Quorum sensing via an N-acyl homoserine lactone (HSL) pheromone controls the biosynthesis of a carbapenem antibiotic in Erwinia carotovora. Transcription of the carbapenem biosynthetic genes is dependent on the LuxR-type activator protein, CarR. Equilibrium binding of a range of HSL molecules, which are thought to activate CarR to bind to its DNA target sequence, was examined using fluorescence quenching, DNA bandshift analysis, limited proteolysis and reporter gene assays. CarR bound the most physiologically relevant ligand, N-(3-oxohexanoyl)-L-homoserine lactone, with a stoichiometry of two molecules of ligand per dimer of protein and a dissociation constant of 1.8 microM, in good agreement with the concentration of HSL required to activate carbapenem production in vivo. In the presence of HSL, CarR formed a very high molecular weight complex with its target DNA, indicating that the ligand causes the protein to multimerize. Chemical cross-linking analysis supported this interpretation. Our data show that the ability of a given HSL to facilitate CarR binding to its target DNA sequence is directly proportional to the affinity of the HSL for the protein.
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