ArticleThe EMBO journal2000
Op18/stathmin caps a kinked protofilament-like tubulin tetramer.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 100 citations in OpenAlex.
- Tubulin regulates stability and localization of STMN2 by binding preferentially to its soluble form.The Journal of cell biology · 2025Article
- Computational Approaches to the Rational Design of Tubulin-Targeting Agents.Biomolecules · 2023Review
- Molecular interactions at the colchicine binding site in tubulin: An X-ray crystallography perspective.Drug discovery today · 2022Review
- The molecular biology of tubulinopathies: Understanding the impact of variants on tubulin structure and microtubule regulation.Frontiers in cellular neuroscience · 2022Review
- Microtubule dynamics: an interplay of biochemistry and mechanics.Nature reviews. Molecular cell biology · 2018Review
- New Insights into the Coupling between Microtubule Depolymerization and ATP Hydrolysis by Kinesin-13 Protein Kif2C.The Journal of biological chemistry · 2015Article
- The contribution of αβ-tubulin curvature to microtubule dynamics.The Journal of cell biology · 2014Review
- Biochemical and structural insights into microtubule perturbation by CopN from Chlamydia pneumoniae.The Journal of biological chemistry · 2014Article
- Design and characterization of modular scaffolds for tubulin assembly.The Journal of biological chemistry · 2012Article
- Characterization and detection of cellular and proteomic alterations in stable stathmin-overexpressing, taxol-resistant BT549 breast cancer cells using offgel IEF/PAGE difference gel electrophoresis.Mutation research · 2011Article
- Drugs that target dynamic microtubules: a new molecular perspective.Medicinal research reviews · 2011Review
- Stathmin and interfacial microtubule inhibitors recognize a naturally curved conformation of tubulin dimers.The Journal of biological chemistry · 2010Article
- Drosophila stathmins bind tubulin heterodimers with high and variable stoichiometries.The Journal of biological chemistry · 2010Article
- Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.Cellular and molecular life sciences : CMLS · 2009Review
- Regulation of microtubule dynamic instability in vitro by differentially phosphorylated stathmin.The Journal of biological chemistry · 2009Article
- The PN2-3 domain of centrosomal P4.1-associated protein implements a novel mechanism for tubulin sequestration.The Journal of biological chemistry · 2009Article
- The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling.Infection and immunity · 2009Article
- SCLIP is crucial for the formation and development of the Purkinje cell dendritic arbor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.Molecular biology of the cell · 2008Article
- Functional anthology of intrinsic disorder. 2. Cellular components, domains, technical terms, developmental processes, and coding sequence diversities correlated with long disordered regions.Journal of proteome research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oncoprotein 18/stathmin (Op18), a regulator of microtubule dynamics, was recombinantly expressed and its structure and function analysed. We report that Op18 by itself can fold into a flexible and extended alpha-helix, which is in equilibrium with a less ordered structure. In complex with tubulin, however, all except the last seven C-terminal residues of Op18 are tightly bound to tubulin. Digital image analysis of Op18:tubulin electron micrographs revealed that the complex consists of two longitudinally aligned alpha/beta-tubulin heterodimers. The appearance of the complex was that of a kinked protofilament-like structure with a flat and a ribbed side. Deletion mapping of Op18 further demonstrated that (i) the function of the N-terminal part of the molecule is to 'cap' tubulin subunits to ensure the specificity of the complex and (ii) the complete C-terminal alpha-helical domain of Op18 is necessary and sufficient for stable Op18:tubulin complex formation. Together, our results suggest that besides sequestering tubulin, the structural features of Op18 enable the protein specifically to recognize microtubule ends to trigger catastrophes.
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