ArticleThe EMBO journal2000
Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 113 citations in OpenAlex.
- Dysregulation of mRNA expression by hsa-miR-186 overexpression in arsenic-induced skin carcinogenesis.Toxicology and applied pharmacology · 2025Article
- Identification of transglutaminase 2 mediated polyaminated proteins in a hepatocellular cancer cell line.Computational and structural biotechnology journal · 2025Article
- Article
- Article
- Review
- Probiotics: If It Does Not Help It Does Not Do Any Harm. Really?Microorganisms · 2019Review
- CNF1-like deamidase domains: common Lego bricks among cancer-promoting immunomodulatory bacterial virulence factors.Pathogens and disease · 2018Review
- Bordetella Pertussis virulence factors in the continuing evolution of whooping cough vaccines for improved performance.Medical microbiology and immunology · 2018Review
- Protein cross-linking by chlorinated polyamines and transglutamylation stabilizes neutrophil extracellular traps.Cell death & disease · 2016Article
- Polymorphisms influencing expression of dermonecrotic toxin in Bordetella bronchiseptica.PloS one · 2015Article
- Polyamines transduce the nongenomic, androgen-induced calcium sensitization in intestinal smooth muscle.Molecular endocrinology (Baltimore, Md.) · 2013Article
- What a difference a Dalton makes: bacterial virulence factors modulate eukaryotic host cell signaling systems via deamidation.Microbiology and molecular biology reviews : MMBR · 2013Review
- Histaminylation of glutamine residues is a novel posttranslational modification implicated in G-protein signaling.FEBS letters · 2012Article
- Cryptococcus neoformans activates RhoGTPase proteins followed by protein kinase C, focal adhesion kinase, and ezrin to promote traversal across the blood-brain barrier.The Journal of biological chemistry · 2012Article
- Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomics.Biology direct · 2012Article
- Type III effector VopC mediates invasion for Vibrio species.Cell reports · 2012Article
- Bacterial protein toxins that modify host regulatory GTPases.Nature reviews. Microbiology · 2011Review
- Targeting of the actin cytoskeleton by insecticidal toxins from Photorhabdus luminescens.Naunyn-Schmiedeberg's archives of pharmacology · 2011Review
- Association of Bordetella dermonecrotic toxin with the extracellular matrix.BMC microbiology · 2010Article
- Recent insights into Pasteurella multocida toxin and other G-protein-modulating bacterial toxins.Future microbiology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The small GTPase Rho, which regulates a variety of cell functions, also serves as a specific substrate for bacterial toxins. Here we demonstrate that Bordetella dermonecrotizing toxin (DNT) catalyzes cross-linking of Rho with ubiquitous polyamines such as putrescine, spermidine and spermine. Mass spectrometric analyses revealed that the cross-link occurred at Gln63, which had been reported to be deamidated by DNT in the absence of polyamines. Rac1 and Cdc42, other members of the Rho family GTPases, were also polyaminated by DNT. The polyamination, like the deamidation, markedly reduced the GTPase activity of Rho without affecting its GTP-binding activity, indicating that polyaminated Rho behaves as a constitutively active analog. Moreover, polyamine-linked Rho, even in the GDP-bound form, associated more effectively with its effector ROCK than deamidated Rho in the GTP-bound form and, when microinjected into cells, induced the anomalous formation of stress fibers indistinguishable from those seen in DNT-treated cells. The results imply that the polyamine-linked Rho, transducing signals to downstream ROCK in a novel GTP-independent manner, plays an important role in DNT cell toxicity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.