ArticleThe Journal of physiology2000
Recycling and refilling of transmitter quanta at the frog neuromuscular junction.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Presynaptic G-protein-coupled receptors regulate synaptic cleft glutamate via transient vesicle fusion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Dynamic regulation of synaptic GABA release by the glutamate-glutamine cycle in hippocampal area CA1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Neuronal glutamate uptake Contributes to GABA synthesis and inhibitory synaptic strength.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Effects of 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one on synaptic vesicle cycling at the frog neuromuscular junction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Regulation of vesicular acetylcholine transporter by the activation of excitatory amino acid receptors in the avian retina.Cellular and molecular neurobiology · 2002Article
- Vesicle size and transmitter release at the frog neuromuscular junction when quantal acetylcholine content is increased or decreased.The Journal of physiology · 2002Article
- Repetitive nerve stimulation decreases the acetylcholine content of quanta at the frog neuromuscular junction.The Journal of physiology · 2001Article
- Presynaptic function is altered in snake K+-depolarized motor nerve terminals containing compromised mitochondria.The Journal of physiology · 2001Article
- Synaptic vesicle transporter expression regulates vesicle phenotype and quantal size.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Fluorescent dyes have been used at the frog neuromuscular junction to label synaptic vesicular membrane. Retrieved membrane is reformed into vesicles, which are released along with pre-existing vesicles. Consequently, if vesicular refilling with acetylcholine (ACh) is depressed by inhibitors, two sizes of quanta should be released: normal and smaller. As recycling continues the fraction of smaller size quanta should increase exponentially. 2. We enhanced the rate of quantal release by elevating the K+ concentration. The principal inhibitors were (-)-vesamicol (VES), hemicholinium-3 (HC3), and NH4+. Quantal size measurements were fitted to one and to two cumulative lognormal probability distribution functions. When two fitted better, the statistical significance assessment took into account the three additional parameters used in calculating the fit. 3. After recycling in the presence of inhibitor, many sets were fitted better by two lognormal functions. As recycling continued, the fraction of the miniature endplate potential voltage-time integrals ( MEPPs) in the larger sub-population decreased exponentially. 4. The size of the releasable pool was estimated by counting the quanta released by carbonyl cyanide m-chlorophenylhydrazone (CCCP). This was compared to pool sizes calculated from the inhibitor experiments. The two estimates of pool size were indistinguishable, with mean values ranging from about 170,000 to 270,000. 5. With all of the treatments tested, the means of the sizes in the smaller sub-population of MEPPs were about 1/3 those of the larger sub-populations. 6. Recycling synaptic vesicles appear to be incorporated into the releasable pool from which they have roughly the same probability of release as the pre-existing vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.