ArticleThe Journal of physiology2000
Mechanically gated channel activity in cytoskeleton-deficient plasma membrane blebs and vesicles from Xenopus oocytes.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 96 citations in OpenAlex.
- TRPV4 and chloride channels mediate volume sensing in trabecular meshwork cells.American journal of physiology. Cell physiology · 2024Article
- Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension.Nature communications · 2016Article
- Expressing and characterizing mechanosensitive channels in Xenopus oocytes.Methods in molecular biology (Clifton, N.J.) · 2015Article
- The force-from-lipid (FFL) principle of mechanosensitivity, at large and in elements.Pflugers Archiv : European journal of physiology · 2015Review
- Article
- Molecular force transduction by ion channels: diversity and unifying principles.Journal of cell science · 2012Review
- Spontaneous excitation patterns computed for axons with injury-like impairments of sodium channels and Na/K pumps.PLoS computational biology · 2012Article
- Chemical-induced pH-mediated molecular switch.Analytical chemistry · 2011Article
- Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force.The Journal of biological chemistry · 2010Article
- Actin cytoskeleton regulates stretch-activated Ca2+ influx in human pulmonary microvascular endothelial cells.American journal of respiratory cell and molecular biology · 2010Article
- Adaptive behavior of bacterial mechanosensitive channels is coupled to membrane mechanics.The Journal of general physiology · 2010Article
- Stretch-activated ion channels: what are they?Physiology (Bethesda, Md.) · 2010Review
- Attachment of osteocyte cell processes to the bone matrix.Anatomical record (Hoboken, N.J. : 2007) · 2009Article
- TRP channels and mechanosensory transduction: insights into the arterial myogenic response.Pflugers Archiv : European journal of physiology · 2008Review
- Revisiting TRPC1 and TRPC6 mechanosensitivity.Pflugers Archiv : European journal of physiology · 2008Article
- Mechanotransduction: touch and feel at the molecular level as modeled in Caenorhabditis elegans.Molecular neurobiology · 2007Review
- Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology.Toxicon : official journal of the International Society on Toxinology · 2007Review
- Liposome reconstitution and modulation of recombinant N-methyl-D-aspartate receptor channels by membrane stretch.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Desensitization of mechano-gated K2P channels.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
1. A novel technique involving hypertonic stress causes membrane 'blebbing' of the Xenopus oocyte and the shedding of plasma membrane vesicles (PMVs). 2. Confocal fluorescence microscopy, immunocytochemistry and electron microscopy indicate that blebs and PMVs lack cortical cytoskeleton and are deficient in cytoskeleton proteins and devoid of microvilli. 3. Patch recordings from PMVs consistently reveal mechanically gated (MG) channel activity. The MG channels display the same single-channel conductance as control recordings but differ in terms of reduced mechanosensitivity and adaptation to sustained stimulation. 4. Whole PMV recordings show rapid and reversible activation of mechanosensitive currents in response to pressure pulses. The maximal currents activated in PMVs are consistent with MG channel activity recorded in patches. 5. The discrepancy between MG channel activity recorded in whole PMVs and oocytes most probably reflects their different membrane geometry and ability to develop activating bilayer tensions. 6. We propose that membrane blebbing, which is known to occur under specific physiological and pathological conditions (e.g. mitosis and apoptosis), may increase mechanosensitivity independently of the intrinsic properties of membrane proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.