ArticleThe Journal of physiology2000
On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Patch-Clamp Analysis of the Mitochondrial HFrontiers in physiology · 2020Article
- Anion permeation in calcium-activated chloride channels formed by TMEM16A from Xenopus tropicalis.Pflugers Archiv : European journal of physiology · 2014Article
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients.European biophysics journal : EBJ · 2011Article
- Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles.PloS one · 2011Article
- Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force.The Journal of biological chemistry · 2010Article
- Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.The Journal of physiology · 2010Article
- Biophysics and structure of the patch and the gigaseal.Biophysical journal · 2009Article
- Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts.European biophysics journal : EBJ · 2009Article
- ATP inhibition of CLC-1 is controlled by oxidation and reduction.The Journal of general physiology · 2008Article
- Turnover rate of the gamma-aminobutyric acid transporter GAT1.The Journal of membrane biology · 2007Article
- Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubes.The Journal of physiology · 2007Article
- The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts.American journal of physiology. Cell physiology · 2007Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Membrane reserves and hypotonic cell swelling.The Journal of membrane biology · 2006Article
- Cell swelling-induced ATP release is tightly dependent on intracellular calcium elevations.The Journal of physiology · 2004Article
- Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes.Molecular and cellular biology · 2004Article
- Membrane stretch accelerates activation and slow inactivation in Shaker channels with S3-S4 linker deletions.Biophysical journal · 2002Article
- Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- An improved method for real-time monitoring of membrane capacitance in Xenopus laevis oocytes.Biophysical journal · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Mechanical stimulation of voltage-clamped Xenopus oocytes by inflation, aspiration, or local indentation failed to activate an increase in membrane conductance up to the point of causing visible oocyte damage. 2. The absence of mechanosensitivity is not due to the vitelline membrane, rapid MG channel adaptation or tension-sensitive recruitment of new membrane. 3. Membrane capacitance measurements indicate that the oocyte surface area is at least 5 times larger than that predicted assuming a smooth sphere. We propose that this excess membrane area provides an immediate reserve that can 'buffer' membrane tension changes and thus prevent MG channel activation. 4. High-resolution images of tightly sealed patches and patch capacitance measurements indicate a smooth membrane that is pulled flat and perpendicular across the inside of the pipette. Brief steps of pressure or suction cause rapid and reversible membrane flexing and MG channel activation. 5. We propose that changes in membrane geometry induced during cell growth and differentiation or as a consequence of specific physiological and pathological conditions may alter mechanosensitivity of a cell independently of the intrinsic properties of channel proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.