ArticleThe Journal of physiology2000
Calcium-, voltage- and osmotic stress-sensitive currents in Xenopus oocytes and their relationship to single mechanically gated channels.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- A hyperpolarization-activated ion current of amphibian oocytes.Pflugers Archiv : European journal of physiology · 2013Article
- Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force.The Journal of biological chemistry · 2010Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Mechanosensitive cation channels in human leukaemia cells: calcium permeation and blocking effect.The Journal of physiology · 2002Article
- Two-dimensional kinetic analysis suggests nonsequential gating of mechanosensitive channels in Xenopus oocytes.Biophysical journal · 2001Article
- Mechanically gated channel activity in cytoskeleton-deficient plasma membrane blebs and vesicles from Xenopus oocytes.The Journal of physiology · 2000Article
- On the discrepancy between whole-cell and membrane patch mechanosensitivity in Xenopus oocytes.The Journal of physiology · 2000Article
- Piezo1: properties of a cation selective mechanical channel.Channels (Austin, Tex.)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Patch recordings from Xenopus oocytes indicated that mechanically gated (MG) channels are expressed at a uniform surface density ( approximately 1 channel microm-2) with an estimated > 3 x 106 MG channels per oocyte that could generate microamps of current at +/-50 mV. 2. Removal of external Ca2+ induced a membrane conductance that differed from MG channels in ion selectivity, pharmacology and sensitivity to connexion-38. 3. Depolarization to +50 mV activated a Na+-selective, a Cl--selective and a non-selective conductance. Hyperpolarization to -150 mV activated a non-selective conductance. None of these conductances appeared to be mediated by MG channels. 4. Hypotonicity (25 %) failed to evoke any change in membrane conductance in the majority of defolliculated oocytes. Hypertonicity (200 %) evoked a large non-selective (PK /PCl approximately 1) membrane conductance that was not blocked by 100 microM Gd3+. 5. Although the above stimuli could activate a variety of whole-oocyte conductances, including three novel conductances, they did not involve MG channel activation. Possible mechanisms underlying the discrepancy between observed conductances and those anticipated from patch-clamp studies are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.