ArticleGut2000
Human intestinal epithelial cells secrete interleukin-1 receptor antagonist and interleukin-8 but not interleukin-1 or interleukin-6.
Article in Gut, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 103 citations in OpenAlex.
- Trial
- From Cytokines to Biomarkers: Mapping the Immunopathology of Inflammatory Bowel Disease.Cells · 2025Review
- A Multicellular In Vitro Model of the Human Intestine with Immunocompetent Features Highlights Host-Pathogen Interactions During Early Salmonella Typhimurium Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Miniature chicken ileal explant culture to investigate the inflammatory response induced by pathogen-associated molecular patterns.Frontiers in veterinary science · 2025Article
- Article
- Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection.PLoS pathogens · 2022Article
- Chemotherapy induced gastrointestinal toxicities.Advances in cancer research · 2022Review
- Development of a self-limiting model of methotrexate-induced mucositis reinforces butyrate as a potential therapy.Scientific reports · 2021Article
- Role of the SOS Response in the Generation of Antibiotic ResistanceAntimicrobial agents and chemotherapy · 2021Article
- Chemotherapeutics-Induced Intestinal Mucositis: Pathophysiology and Potential Treatment Strategies.Frontiers in pharmacology · 2021Review
- Characterization of Chenopodin Isoforms from Quinoa Seeds and Assessment of Their Potential Anti-Inflammatory Activity in Caco-2 Cells.Biomolecules · 2020Article
- Human Gut-Associated Natural Killer Cells in Health and Disease.Frontiers in immunology · 2019Review
- Bacteriophages targeting intestinal epithelial cells: a potential novel form of immunotherapy.Cellular and molecular life sciences : CMLS · 2018Article
- Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line.World journal of gastroenterology · 2017Article
- Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells.Mucosal immunology · 2017Article
- Cytokines and growth factors in the developing intestine and during necrotizing enterocolitis.Seminars in perinatology · 2017Review
- Immunologic and Hematological Abnormalities in Necrotizing Enterocolitis.Clinics in perinatology · 2015Review
- Effects of ex-vivo and in-vivo treatment with probiotics on the inflammasome in dogs with chronic enteropathy.PloS one · 2015Article
- Serum IL-17 and IL-6 increased accompany with TGF-β and IL-13 respectively in ulcerative colitis patients.International journal of clinical and experimental medicine · 2014Article
- Opposing Functions of Classic and Novel IL-1 Family Members in Gut Health and Disease.Frontiers in immunology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is growing evidence that intestinal epithelial cells (IECs) are involved in the mucosal immune system.
aimTo assess the pattern of cytokines secreted by IECs and lamina propria mononuclear cells (LPMNCs). To achieve this, the expression and secretion of interleukin (IL)-1, IL-1 receptor antagonist (IL-1ra), IL-6, and IL-8 in human primary colonic and ileal IECs and LPMNCs from the same patient were studied.
methodsIECs and LPMNCs were isolated from surgical specimens or endoscopic biopsy samples. mRNA expression was investigated by northern blot analysis. Secretion of IL-1beta, IL-6, IL-8, and IL-1ra was measured by enzyme linked immunosorbent assay.
resultsIL-1ra mRNA levels were higher in IECs than in LPMNCs in all probands. IL-8 mRNA was only present in low amounts in the IECs from two controls. In none of the specimens were IL-1beta and IL-6 mRNA present in IECs. Transcripts encoding IL-1beta, IL-1ra, IL-6, and IL-8 were identified in LPMNC preparations of all specimens. IECs from normal mucosa produced no detectable amounts of IL-1beta or IL-6, whereas LPMNCs did. IECs secreted some IL-8 (65 (9) pg/10(5) cells) but significantly more was generated by LPMNCs (408 (43) pg/10(5) cells, p<0.0001). However, IECs secreted more IL-1ra than did LPMNCs (120 (12) v 94 (11) pg/10(5) cells). In acute inflammation, IEC IL-1ra secretion was significantly increased. A correlation between secreted IL-1ra and the macroscopical degree of inflammation was found in Crohn's disease (r = 0.64, p<0.0001, n = 36) and ulcerative colitis (r = 0. 76, p<0.0001, n = 24).
conclusionsIECs from normal mucosa express and secrete IL-1ra and low amounts of IL-8, but no IL-1 or IL-6. In inflamed mucosa the secretion of IL-1ra by IECs is slightly increased but may be not sufficient to antagonise the greatly increased production of proinflammatory cytokines by LPMNCs and the IECs themselves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.