ArticleThe Journal of biological chemistry2000
Cloning and expression of glycolipid transfer protein from bovine and porcine brain.
Article in The Journal of biological chemistry, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Use of Bicelle-Generated Lipid Bilayer Vesicle Nanoparticles for In Vitro Measurement of Lipid Intermembrane Transport.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Tandem Mass Tagging-Based Quantitative Proteomics Analysis Reveals Damage to the Liver and Brain ofAntioxidants (Basel, Switzerland) · 2022Article
- Review
- Emerging roles for human glycolipid transfer protein superfamily members in the regulation of autophagy, inflammation, and cell death.Progress in lipid research · 2020Review
- Measuring Lipid Transfer Protein Activity Using Bicelle-Dilution Model Membranes.Analytical chemistry · 2020Article
- Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- How α-Helical Motifs Form Functionally Diverse Lipid-Binding Compartments.Annual review of biochemistry · 2017Review
- Phosphatidylserine Stimulates Ceramide 1-Phosphate (C1P) Intermembrane Transfer by C1P Transfer Proteins.The Journal of biological chemistry · 2017Article
- Sphingolipid transfer proteins defined by the GLTP-fold.Quarterly reviews of biophysics · 2015Article
- Human glycolipid transfer protein gene (GLTP) expression is regulated by Sp1 and Sp3: involvement of the bioactive sphingolipid ceramide.The Journal of biological chemistry · 2011Article
- Gene expression profiling of oral squamous cell carcinoma by differential display rt-PCR and identification of tumor biomarkers.Indian journal of surgical oncology · 2010Article
- Human GLTP: Three distinct functions for the three tryptophans in a novel peripheral amphitropic fold.Biophysical journal · 2010Article
- Structural determination and tryptophan fluorescence of heterokaryon incompatibility C2 protein (HET-C2), a fungal glycolipid transfer protein (GLTP), provide novel insights into glycolipid specificity and membrane interaction by the GLTP fold.The Journal of biological chemistry · 2010Article
- Structural basis of wedging the Golgi membrane by FAPP pleckstrin homology domains.EMBO reports · 2010Article
- Human GLTP and mutant forms of ACD11 suppress cell death in the Arabidopsis acd11 mutant.The FEBS journal · 2008Article
- Human glycolipid transfer protein (GLTP) genes: organization, transcriptional status and evolution.BMC genomics · 2008Article
- Glycolipid transfer proteins.Biochimica et biophysica acta · 2007Review
- The liganding of glycolipid transfer protein is controlled by glycolipid acyl structure.PLoS biology · 2006Article
- Glycolipid transfer protein interaction with bilayer vesicles: modulation by changing lipid composition.Biophysical journal · 2005Article
- Point mutational analysis of the liganding site in human glycolipid transfer protein. Functionality of the complex.The Journal of biological chemistry · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Glycolipid transfer protein (GLTP) is a small (23-24 kDa), basic protein (pI congruent with 9.0) that accelerates the intermembrane transfer of various glycolipids. Here, we report the first cloning of cDNAs that encode the bovine and porcine GLTPs. The cDNA open reading frame for bovine GLTP was constructed by bridge-overlapping extension polymerase chain reaction (PCR) after obtaining partial coding cDNA clones by hot start, seminested, and rapid amplification of cDNA ends-PCR. The cDNA open reading frame for porcine GLTP was constructed by reverse transcriptase-PCR. The encoded amino acid sequences in the full-length bovine and porcine cDNAs were identical, consisting of 209 amino acid residues, and were nearly the same as the published sequence determined by Edman degradation. The cDNA encoded one additional amino acid at the N terminus (methionine), arginine at positions 10 and 200 instead of lysine, and threonine at position 65 instead of alanine. Expression of GLTP-cDNA in Escherichia coli using pGEX-6P-1 vector resulted in glutathione S-transferase (GST)-GLTP fusion protein. Regulation of growth and induction conditions led to approximately 50% of expressed fusion protein being soluble and active. Proteolytic cleavage of GST-GLTP fusion protein (bound to GST-Sepharose) and affinity purification resulted in fully active GLTP. Northern blot analyses of bovine tissues showed a single transcript of approximately 2.2 kilobases and the following hierarchy of mRNA levels: cerebrum > kidney > spleen congruent with lung congruent with cerebellum > liver > heart muscle. Reverse transcriptase-PCR analyses of mRNA levels supported the Northern blot results.
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