ArticleMolecular and cellular biology2000
Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Review
- Fidelity, specialization, and evolution of Paramecium PolX DNA polymerases involved in programmed double-strand break DNA repair.Nucleic acids research · 2025Article
- Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle.Nucleic acids research · 2023Article
- The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases.Mobile DNA · 2021Article
- The Paramecium histone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements.PLoS genetics · 2020Article
- Functional diversification of Paramecium Ku80 paralogs safeguards genome integrity during precise programmed DNA elimination.PLoS genetics · 2020Article
- Programmed genome rearrangements in Oxytricha produce transcriptionally active extrachromosomal circular DNA.Nucleic acids research · 2019Article
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- RNA-mediated transgenerational inheritance in ciliates and plants.Chromosoma · 2018Review
- Multimerization properties of PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements.Nucleic acids research · 2017Article
- DNA deletion as a mechanism for developmentally programmed centromere loss.Nucleic acids research · 2016Article
- Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.Nucleic acids research · 2015Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination.Nucleic acids research · 2014Article
- Local effect of enhancer of zeste-like reveals cooperation of epigenetic and cis-acting determinants for zygotic genome rearrangements.PLoS genetics · 2014Article
- Ku-mediated coupling of DNA cleavage and repair during programmed genome rearrangements in the ciliate Paramecium tetraurelia.PLoS genetics · 2014Article
- Is non-homologous end-joining really an inherently error-prone process?PLoS genetics · 2014Review
- Spliced DNA sequences in the Paramecium germline: their properties and evolutionary potential.Genome biology and evolution · 2013Article
- The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paramecium internal eliminated sequences (IESs) are short AT-rich DNA elements that are precisely eliminated from the germ line genome during development of the somatic macronucleus. They are flanked by one 5'-TA-3' dinucleotide on each side, a single copy of which remains at the donor site after excision. The timing of their excision was examined in synchronized conjugating cells by quantitative PCR. Significant amplification of the germ line genome was observed prior to IES excision, which starts 12 to 14 h after initiation of conjugation and extends over a 2- to 4-h period. Following excision, two IESs were shown to form extrachromosomal circles that can be readily detected on Southern blots of genomic DNA from cells undergoing macronuclear development. On these circular molecules, covalently joined IES ends are separated by one copy of the flanking 5'-TA-3' repeat. The similar structures of the junctions formed on the excised and donor molecules point to a central role for this dinucleotide in IES excision.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.