ArticleRNA (New York, N.Y.)2000
Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Targeting PUF60 prevents tumor progression by retarding mRNA decay of oxidative phosphorylation in ovarian cancer.Cellular oncology (Dordrecht, Netherlands) · 2024Article
- Compendium of Methods to Uncover RNA-Protein Interactions In Vivo.Methods and protocols · 2021Review
- The Bacterial Ro60 Protein and Its Noncoding Y RNA Regulators.Annual review of microbiology · 2020Review
- Noncoding Y RNAs regulate the levels, subcellular distribution and protein interactions of their Ro60 autoantigen partner.Nucleic acids research · 2020Article
- Y RNA: An Overview of Their Role as Potential Biomarkers and Molecular Targets in Human Cancers.Cancers · 2020Review
- Ro60 and Y RNAs: structure, functions, and roles in autoimmunity.Critical reviews in biochemistry and molecular biology · 2019Review
- Bacterial Y RNAs: Gates, Tethers, and tRNA Mimics.Microbiology spectrum · 2018Review
- Circulating Y-RNAs in Extracellular Vesicles and Ribonucleoprotein Complexes; Implications for the Immune System.Frontiers in immunology · 2018Review
- PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren's syndrome.Annals of the rheumatic diseases · 2016Article
- DASHR: database of small human noncoding RNAs.Nucleic acids research · 2016Article
- Functional roles of non-coding Y RNAs.The international journal of biochemistry & cell biology · 2015Review
- Non-coding Y RNAs as tethers and gates: Insights from bacteria.RNA biology · 2013Review
- Article
- Why YRNAs? About Versatile RNAs and Their Functions.Biomolecules · 2013Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA.RNA (New York, N.Y.) · 2012Article
- The subcellular distribution of an RNA quality control protein, the Ro autoantigen, is regulated by noncoding Y RNA binding.Molecular biology of the cell · 2009Article
- Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control.Genes & development · 2007Article
- Article
- Retropseudogenes derived from the human Ro/SS-A autoantigen-associated hY RNAs.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human Ro ribonucleoproteins (RNPs) are autoantigenic particles of unknown function(s) that consist of a 60-kDa protein (Ro60) associated with one hY RNA (hY1-5). Using a modified yeast three-hybrid system, named RNP interaction trap assay (RITA), we cloned a novel Ro RNP-binding protein (RoBPI), based on its property to interact in vivo in yeast with an RNP complex made of recombinant Ro60 (rRo60) protein and hY5 (rhY5) RNA. RoBPI cDNA contains three conserved RNA recognition motifs (RRM) and is present as a family of isoforms differing slightly at their 5' end. The 2.0-kb RoBPI mRNA was detected in all human tissues tested. Highly homologous cDNA sequences were found in banks of expressed sequence tags (ESTs) from mice. Two-hybrid, three-hybrid, and RITA experiments respectively established that 60 kDa RoBPI did not interact in yeast with rRo60 alone, with rhY5 RNA alone, or with bait RNPs consisting of rRo60 and recombinant hY1, hY3, or hY4 RNAs. RoBPI coimmunoprecipitated with Ro RNPs from HeLa cell extracts and partially colocalized with Ro60 in nuclei of cultured cells. Because hY5 RNA and RohY5 RNPs are recent evolutionary additions seen only in primates, but RoBPI seems more conserved, their interaction may represent a gain of function for Ro RNPs. Alternatively, interaction of RohY5 RNPs with RoBPI may have no functional bearing, but may underlie some of the unique biochemical and immunological properties of these RNPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.