ArticleRNA (New York, N.Y.)2000
A DNA target of 30 bp is sufficient for RNA-directed DNA methylation.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 119 citations in OpenAlex.
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Empowering plant epigenetics to breed resilience of crops: From nucleolar dominance to transgenerational epigenetic inheritance.The plant genome · 2025Review
- Transgenerational epigenetic inheritance during plant evolution and breeding.Trends in plant science · 2024Review
- Small RNAs mediate transgenerational inheritance of genome-wide trans-acting epialleles in maize.Genome biology · 2022Article
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Review
- Comprehensive Mechanism of Gene Silencing and Its Role in Plant Growth and Development.Frontiers in plant science · 2021Review
- Epigenetic Modifications: An Unexplored Facet of Exogenous RNA Application in Plants.Plants (Basel, Switzerland) · 2020Article
- Maternal small RNAs mediate spatial-temporal regulation of gene expression, imprinting, and seed development inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Innate Immunity Activation and RNAi Interplay in Citrus Exocortis Viroid-Tomato Pathosystem.Viruses · 2018Article
- Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin.eLife · 2016Article
- Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein.Scientific reports · 2016Article
- A nuclear-replicating viroid antagonizes infectivity and accumulation of a geminivirus by upregulating methylation-related genes and inducing hypermethylation of viral DNA.Scientific reports · 2016Article
- Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA.RNA biology · 2015Article
- SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation.Nature · 2014Article
- The role of methylation of DNA in environmental adaptation.Integrative and comparative biology · 2013Article
- Interplay among RNA polymerases II, IV and V in RNA-directed DNA methylation at a low copy transgene locus in Arabidopsis thaliana.Plant molecular biology · 2013Article
- Transgenerational maintenance of transgene body CG but not CHG and CHH methylation.Epigenetics · 2012Article
- Male germline control of transposable elements.Biology of reproduction · 2012Review
- A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco.Plant molecular biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher plants, RNA-DNA interactions can trigger de novo methylation of genomic sequences via a process that is termed RNA-directed DNA methylation (RdDM). In potato spindle tuber viroid (PSTVd)-infected tobacco plants, this process can potentially lead to methylation of all C residues at symmetrical and nonsymmetrical sites within chromosomal inserts that consist of multimers of the 359-bp-long PSTVd cDNA. Using PSTVd cDNA subfragments, we found that genomic targets with as few as 30 nt of sequence complementarity to the viroid RNA are detected and methylated. Genomic sequencing analyses of genome-integrated 30- and 60-bp-long PSTVd subfragments demonstrated that de novo cytosine methylation is not limited to the canonical CpG, CpNpG sites. Sixty-base-pair-long PSTVd cDNA constructs appeared to be densely methylated in nearly all tobacco leaf cells. With the 30-bp-long PSTVd-specific construct, the proportion of cells displaying dense transgene methylation was significantly reduced, suggesting that a minimal target size of about 30 bp is necessary for RdDM. The methylation patterns observed for two different 60-bp constructs further suggested that the sequence identity of the target may influence the methylation mechanism. Finally, a link between viroid pathogenicity and PSTVd RNA-directed methylation of host sequences is proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.