ArticleNucleic acids research2000
Poly(A)-binding protein I of Leishmania: functional analysis and localisation in trypanosomatid parasites.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Poly(A)-binding proteins in trypanosomatids and Plasmodium.Journal of biosciences · 2026Article
- Knockout of protein phosphatase 1 in Leishmania major reveals its role during RNA polymerase II transcription termination.Nucleic acids research · 2023Article
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Regulation of Translation in the Protozoan ParasiteInternational journal of molecular sciences · 2020Review
- Comparative proteomics of the two T. brucei PABPs suggests that PABP2 controls bulk mRNA.PLoS neglected tropical diseases · 2018Article
- Phosphorylation and interactions associated with the control of the Leishmania Poly-A Binding Protein 1 (PABP1) function during translation initiation.RNA biology · 2018Article
- Molecular and Functional Characterization of ssDNA Aptamers that Specifically Bind Leishmania infantum PABP.PloS one · 2015Article
- Two splicing factors carrying serine-arginine motifs, TSR1 and TSR1IP, regulate splicing, mRNA stability, and rRNA processing in Trypanosoma brucei.RNA biology · 2014Article
- Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- Evolutionary conservation and diversification of the translation initiation apparatus in trypanosomatids.Comparative and functional genomics · 2012Article
- Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners.Eukaryotic cell · 2010Article
- Evolutionary changes in the Leishmania eIF4F complex involve variations in the eIF4E-eIF4G interactions.Nucleic acids research · 2009Article
- RNA-binding domain proteins in Kinetoplastids: a comparative analysis.Eukaryotic cell · 2005Article
- Presence of a poly(A) binding protein and two proteins with cell cycle-dependent phosphorylation in Crithidia fasciculata mRNA cycling sequence binding protein II.Eukaryotic cell · 2004Article
- Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p.RNA (New York, N.Y.) · 2004Article
- Evidence that poly(A) binding protein has an evolutionarily conserved function in facilitating mRNA biogenesis and export.RNA (New York, N.Y.) · 2003Article
- Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts.Nucleic acids research · 2002Article
- Life without transcriptional control? From fly to man and back again.The EMBO journal · 2002Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of gene expression in trypanosomatid parasites is predominantly post-transcriptional. Primary transcripts are trans-spliced and polyadenylated to generate mature mRNAs and transcript stability is a major factor controlling stage-specific gene expression. Degenerate PCR has been used to clone the gene encoding the Leishmania homologue of poly(A)-binding protein (Lm PAB1), as an approach to the identification of trans-acting factors involved in this atypical mode of eukaryotic gene expression. lmpab1 is a single copy gene encoding a 63 kDa protein which shares major structural features but only 35-40% amino acid identity with other PAB1 sequences, including those of other trypanosomatids. Lm PAB1 is expressed at constant levels during parasite differentiation and is phosphorylated in vivo. It is localised predominantly in the cytoplasm but inhibition of transcription with actinomycin D also reveals diffuse localisation in the nucleus. Lm PAB1 binds poly(A) with high specificity and affinity but fails to complement a null mutation in Saccharomyces cerevisiae. These properties are indicative of functional divergence in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.