ArticleNucleic acids research2000
Transcripts of the ndhH-D operon of barley plastids: possible role of unedited site III in splicing of the ndhA intron.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Recombination and Retroprocessing in Broomrapes Reveal RNA-Mediated Gene Transfer Mechanism and a Generalizable Model for Mitochondrial Evolution in Heterotrophic Plants.Genome biology and evolution · 2026Article
- First Plastome Sequences of Two Endemic Taxa ofBiology · 2026Article
- Loss of plastidComputational and structural biotechnology journal · 2023Article
- Extensive reorganization of the chloroplast genome ofFrontiers in plant science · 2022Article
- Evolution and Function of the Chloroplast. Current Investigations and Perspectives.International journal of molecular sciences · 2018Article
- Dynamics of Chloroplast Translation during Chloroplast Differentiation in Maize.PLoS genetics · 2016Article
- The PPR-SMR protein PPR53 enhances the stability and translation of specific chloroplast RNAs in maize.The Plant journal : for cell and molecular biology · 2016Article
- Deletions in cox2 mRNA result in loss of splicing and RNA editing and gain of novel RNA editing sites.PloS one · 2013Article
- Balanced gene losses, duplications and intensive rearrangements led to an unusual regularly sized genome in Arbutus unedo chloroplasts.PloS one · 2013Article
- Activity, polypeptide and gene identification of thylakoid Ndh complex in trees: potential physiological relevance of fluorescence assays.Physiologia plantarum · 2012Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Specific function of a plastid sigma factor for ndhF gene transcription.Nucleic acids research · 2005Article
- Topology of the plastid Ndh complex and its NDH-F subunit in thylakoid membranes.The Biochemical journal · 2004Article
- Developmental co-variation of RNA editing extent of plastid editing sites exhibiting similar cis-elements.Nucleic acids research · 2003Article
- Transcript abundance supercedes editing efficiency as a factor in developmental variation of chloroplast gene expression.RNA (New York, N.Y.) · 2002Article
- Heterologous, splicing-dependent RNA editing in chloroplasts: allotetraploidy provides trans-factors.The EMBO journal · 2001Article
- Hydrogen peroxide mediates the induction of chloroplastic Ndh complex under photooxidative stress in barley.Plant physiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastid ndhH-D operon produces several transcripts containing ndhA sequence with and without its group II intron. After sequencing an 8125 bp fragment of barley plastid DNA including the ndhH-D operon, we investigated the editing-splicing status of transcripts in the range 1.0-7.8 kb. Reverse transcription and sequencing of RNA bands separated by electrophoresis were used to determine C-->U editing sites. Sites I, II and IV of ndhA and site V of ndhD were edited in all transcripts analysed and, probably, were edited before any splicing had taken place. In contrast, site III of ndhA (13 bp from the 5'-end base of the second exon) was not edited in transcripts containing the intron (including the 1.7 kb intermediary transcript consisting of the intron and the second exon) but was edited in all transcripts lacking the ndhA intron. Comparison of the secondary structures of the ndhA intron and intron-second exon intermediate suggests that G pairing prevents editing of site III in transcripts containing the intron and maintains the secondary structure required for splicing. Splicing of the ndhA intron releases the site III C from pairing and, probably, brings it close to cis-acting elements for editing upstream in the first exon.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.