ArticleJournal of virology2000
The decreased replicative capacity of simian immunodeficiency virus SIVmac239Delta(nef) is manifest in cultures of immature dendritic cellsand T cells.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Halophilic ArchaeaFrontiers in immunology · 2022Article
- PolyICLC Exerts Pro- and Anti-HIV Effects on the DC-T Cell Milieu In Vitro and In Vivo.PloS one · 2016Article
- Adenovirus-based vaccines against rhesus lymphocryptovirus EBNA-1 induce expansion of specific CD8+ and CD4+ T cells in persistently infected rhesus macaques.Journal of virology · 2014Article
- Simian immunodeficiency virus interactions with macaque dendritic cells.Advances in experimental medicine and biology · 2013Review
- Modulation of HIV pathogenesis and T-cell signaling by HIV-1 Nef.Future virology · 2012Article
- Mobilization of HIV spread by diaphanous 2 dependent filopodia in infected dendritic cells.PLoS pathogens · 2012Article
- Attachment and fusion inhibitors potently prevent dendritic cell-driven HIV infection.Journal of acquired immune deficiency syndromes (1999) · 2011Article
- Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo.Retrovirology · 2009Article
- Article
- Macrophage Tropism and Cytopathicity of HIV-1 Variants Isolated Sequentially from a Long-Term Survivor Infected with nef-Deleted Virus.The open microbiology journal · 2007Article
- Transcriptional activity of blood-and cerebrospinal fluid-derived nef/long-terminal repeat sequences isolated from a slow progressor infected with nef-deleted human immunodeficiency virus type 1 (HIV-1) who developed HIV-associated dementia.Journal of neurovirology · 2006Article
- Longitudinal analysis of human immunodeficiency virus type 1 nef/long terminal repeat sequences in a cohort of long-term survivors infected from a single source.Journal of virology · 2006Article
- Covert human immunodeficiency virus replication in dendritic cells and in DC-SIGN-expressing cells promotes long-term transmission to lymphocytes.Journal of virology · 2005Article
- Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef.Journal of virology · 2005Article
- HIV-1 and the hijacking of dendritic cells: a tug of war.Springer seminars in immunopathology · 2005Review
- Human immunodeficiency virus type 1 Nef activates p21-activated kinase via recruitment into lipid rafts.Journal of virology · 2004Article
- Expression of simian immunodeficiency virus (SIV) nef in astrocytes during acute and terminal infection and requirement of nef for optimal replication of neurovirulent SIV in vitro.Journal of virology · 2003Article
- nef gene is required for robust productive infection by simian immunodeficiency virus of T-cell-rich paracortex in lymph nodes.Journal of virology · 2003Article
- Increased macrophage infection upon subcutaneous inoculation of rhesus macaques with simian immunodeficiency virus-loaded dendritic cells or T cells but not with cell-free virus.Journal of virology · 2002Article
- The simian immunodeficiency virus deltaNef vaccine, after application to the tonsils of Rhesus macaques, replicates primarily within CD4(+) T cells and elicits a local perforin-positive CD8(+) T-cell response.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Transmission of simian immunodeficiency virus SIVmac239Delta(nef) (Delta(nef)) to macaques results in attenuated replication of the virus in most animals and ultimately induces protection against challenge with some pathogenic, wild-type SIV strains. It has been difficult, however, to identify a culture system in which the replication of Delta(nef) is severely reduced relative to that of the wild type. We have utilized a primary culture system consisting of blood-derived dendritic cells (DCs) and autologous T cells. When the DCs were fully differentiated or mature, the DC-CD4(+) T-cell mixtures supported replication of both the parental SIV strain, 239 (the wild type), and its mutant with nef deleted (Delta(nef)), irrespective of virus dose and the cell type introducing the virus to the coculture. In contrast, when immature DCs were exposed to Delta(nef) and cocultured with T cells, virus replication was significantly lower than that of the wild type. Activation of the cultures with a superantigen allowed both Delta(nef) and the wild type to replicate comparably in immature DC-T-cell cultures. Immature DCs, which, it has been hypothesized, capture and transmit SIV in vivo, are deficient in supporting replication of Delta(nef) in vitro and may contribute to the reduced pathogenicity of Delta(nef) in vivo.
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