ArticleJournal of virology2000
A stable HeLa cell line that inducibly expresses poliovirus 2A(pro): effects on cellular and viral gene expression.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Calicivirus Non-structural Proteins: Potential Functions in Replication and Host Cell Manipulation.Frontiers in microbiology · 2021Review
- Translational control during poxvirus infection.Wiley interdisciplinary reviews. RNA · 2019Review
- Poxviruses: Slipping and sliding through transcription and translation.PLoS pathogens · 2017Review
- A researcher's guide to the galaxy of IRESs.Cellular and molecular life sciences : CMLS · 2017Review
- Cleavage of Grb2-Associated Binding Protein 2 by Viral Proteinase 2A duringFrontiers in cellular and infection microbiology · 2017Article
- HIV-1 transcripts use IRES-initiation under conditions where Cap-dependent translation is restricted by poliovirus 2A protease.PloS one · 2014Article
- Granzyme B inhibits vaccinia virus production through proteolytic cleavage of eukaryotic initiation factor 4 gamma 3.PLoS pathogens · 2011Article
- The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.Journal of biomedicine & biotechnology · 2011Article
- HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage.PloS one · 2009Article
- Bypass suppression of small-plaque phenotypes by a mutation in poliovirus 2A that enhances apoptosis.Journal of virology · 2009Article
- Proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells.Journal of virology · 2009Article
- Eukaryotic translation initiation factor 4F architectural alterations accompany translation initiation factor redistribution in poxvirus-infected cells.Molecular and cellular biology · 2008Article
- Translational control by viral proteinases.Virus research · 2006Review
- Blockade of the poliovirus-induced cytopathic effect in neural cells by monoclonal antibody against poliovirus or the human poliovirus receptor.Journal of virology · 2005Article
- Regulation of ribonucleotide reductase M2 expression by the upstream AUGs.Nucleic acids research · 2005Article
- Efficient cleavage of ribosome-associated poly(A)-binding protein by enterovirus 3C protease.Journal of virology · 2002Article
- Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro).Journal of virology · 2002Article
- Rous sarcoma virus translation revisited: characterization of an internal ribosome entry segment in the 5' leader of the genomic RNA.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A HeLa cell clone (2A7d) that inducibly expresses the gene for poliovirus protease 2A (2A(pro)) under the control of tetracycline has been obtained. Synthesis of 2A(pro) induces severe morphological changes in 2A7d cells. One day after tetracycline removal, cells round up and a few hours later die. Poliovirus 2A(pro) cleaves both forms of initiation factor eIF4G, causing extensive inhibition of capped-mRNA translation a few hours after protease induction. Methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone, a selective inhibitor of 2A(pro), prevents both eIF4G cleavage and inhibition of translation but not cellular death. Expression of 2A(pro) still allows both the replication of poliovirus and the translation of mRNAs containing a picornavirus leader sequence, while vaccinia virus replication is drastically inhibited. Translation of transfected capped mRNA is blocked in 2A7d-On cells, while luciferase synthesis from a mRNA bearing a picornavirus internal ribosome entry site (IRES) sequence is enhanced by the presence of 2A(pro). Moreover, synthesis of 2A(pro) in 2A7d cells complements the translational defect of a poliovirus 2A(pro)-defective variant. These results show that poliovirus 2A(pro) expression mimics some phenotypical characteristics of poliovirus-infected cells, such as cell rounding, inhibition of protein synthesis and enhancement of IRES-driven translation. This cell line constitutes a useful tool to further analyze 2A(pro) functions, to complement poliovirus 2A(pro) mutants, and to test antiviral compounds.
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