Evidence map›Paper›PMID 10666236›Full record

ArticleJournal of virology2000

Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.

O Guerra-Peraza, M de Tapia, T Hohn, M Hemmings-Mieszczak

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
9.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Review
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  12. Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002
    Review
  13. Ribosome shunt is essential for infectivity of cauliflower mosaic virus.Proceedings of the National Academy of Sciences of the United States of America · 2001
    Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

O Guerra-PerazaFriedrich Miescher-Institut, CH-4002 Basel, Switzerland.
M de Tapia
T Hohn
M Hemmings-Mieszczak
Friedrich Miescher Institute · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using the yeast three-hybrid system, the interaction of the Cauliflower mosaic virus (CaMV) pregenomic 35S RNA (pgRNA) leader with the viral coat protein, its precursor, and a series of derivatives was studied. The purine-rich domain in the center of the pgRNA leader was found to specifically interact with the coat protein. The zinc finger motif of the coat protein and the preceding basic domain were essential for this interaction. Removal of the N-terminal portion of the basic domain led to loss of specificity but did not affect the strength of the interaction. Mutations of the zinc finger motif abolished not only the interaction with the RNA but also viral infectivity. In the presence of the very acidic C-terminal domain, which is part of the preprotein but is not present in the mature CP, the interaction with the RNA was undetectable.

Indexed as

5' Untranslated RegionsBase SequenceCapsidCaulimovirusDNA PrimersMolecular Sequence DataMutationNucleic Acid ConformationNucleic Acid HybridizationOpen Reading FramesRNA, MessengerRNA, Spliced LeaderRNA, ViralYeastsZinc Fingers5' Untranslated RegionsDNA PrimersRNA, MessengerRNA, Spliced LeaderRNA, Viral

Identifiers

PMID10666236
PMCPMC111687
OpenAlexW2114594880

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.