ArticleThe Plant cell2000
A new family of high-affinity transporters for adenine, cytosine, and purine derivatives in Arabidopsis.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 226 citations in OpenAlex.
- Functional identification of key enzymes in calycosin biosynthesis inPharmaceutical science advances · 2026Article
- Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs.Plant & cell physiology · 2026Article
- UPLC-ESI-QTRAP-MS-Based Metabolomics Revealed Changes in Biostimulant-Related Metabolite Profiles inMetabolites · 2025Article
- Structural basis of cytokinin transport by the Arabidopsis AZG2.Nature communications · 2025Article
- Purine-based infochemicals and immunometabolites: a comparative review of emerging signaling pathways in plants and animals.FEMS microbiology reviews · 2025Review
- Nitric oxide and cytokinin cross-talk and their role in plant hypoxia response.Plant signaling & behavior · 2024Review
- The cytokinin efflux transporter ABCC4 participates in Arabidopsis root system development.Plant physiology · 2024Article
- Spatially distributed cytokinins: Metabolism, signaling, and transport.Plant communications · 2024Review
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis.Frontiers in plant science · 2024Article
- SWEET11b transports both sugar and cytokinin in developing barley grains.The Plant cell · 2023Article
- Multi-Knock-a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants.Nature plants · 2023Article
- Plant Hormonomics: A Key Tool for Deep Physiological Phenotyping to Improve Crop Productivity.Plant & cell physiology · 2023Review
- Uncovering transcriptional reprogramming during callus development in soybean: insights and implications.Frontiers in plant science · 2023Article
- Auxin-Cytokinin Cross Talk in Somatic Embryogenesis ofPlants (Basel, Switzerland) · 2022Article
- Phosphate-deprivation and damage signalling by extracellular ATP.Frontiers in plant science · 2022Article
- Functional identification of purine permeases reveals their roles in caffeine transport in tea plants (Frontiers in plant science · 2022Article
- Review
- Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?International journal of molecular sciences · 2021Review
- The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction.Biomolecules · 2021Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many organisms, including plants, nucleic acid bases and derivatives such as caffeine are transported across the plasma membrane. Cytokinins, important hormones structurally related to adenine, are produced mainly in root apices, from where they are translocated to shoots to control a multitude of physiological processes. Complementation of a yeast mutant deficient in adenine uptake (fcy2) with an Arabidopsis cDNA expression library enabled the identification of a gene, AtPUP1 (for Arabidopsis thaliana purine permease1), belonging to a large gene family (AtPUP1 to AtPUP15) encoding a new class of small, integral membrane proteins. AtPUP1 transports adenine and cytosine with high affinity. Uptake is energy dependent, occurs against a concentration gradient, and is sensitive to protonophores, potentially indicating secondary active transport. Competition studies show that purine derivatives (e.g., hypoxanthine), phytohormones (e.g., zeatin and kinetin), and alkaloids (e.g., caffeine) are potent inhibitors of adenine and cytosine uptake. Inhibition by cytokinins is competitive (competitive inhibition constant K(i) = 20 to 35 microM), indicating that cytokinins are transported by this system. AtPUP1 is expressed in all organs except roots, indicating that the gene encodes an uptake system for root-derived nucleic acid base derivatives in shoots or that it exports nucleic acid base analogs from shoots by way of the phloem. The other family members may have different affinities for nucleic acid bases, perhaps functioning as transporters for nucleosides, nucleotides, and their derivatives.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.